School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China.
Department of Obstetrics and Gynecology, Shanghai East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, PR China.
Microb Genom. 2022 Jun;8(6). doi: 10.1099/mgen.0.000848.
Emerging evidence has identified viral circular RNAs (circRNAs) in human cells infected by viruses, interfering with the immune system and inducing diseases including human cancer. However, the biogenesis and regulatory mechanisms of virus-encoded circRNAs in host cells remain unknown. In this study, we used the circRNA detection tool CIRI2 to systematically determine the virus-encoded circRNAs in virus-infected cancer cell lines and cancer patients, by analysing RNA-Seq datasets derived from RNase R-treated samples. Based on the thousands of viral circRNAs we identified, the biological characteristics and potential roles of viral circRNAs in regulating host cell function were determined. In addition, we developed a Viral-circRNA Database (http://www.hywanglab.cn/vcRNAdb/), which is open to all users to search, browse and download information on circRNAs encoded by viruses upon infection.
新出现的证据表明，病毒感染的人类细胞中存在病毒环状 RNA（circRNA），这些 RNA 能够干扰免疫系统并引发疾病，包括人类癌症。然而，宿主细胞中病毒编码的 circRNA 的生物发生和调控机制尚不清楚。在这项研究中，我们使用 circRNA 检测工具 CIRI2，通过分析来自 RNase R 处理样本的 RNA-Seq 数据集，系统地确定了病毒感染的癌细胞系和癌症患者中的病毒编码 circRNA。基于我们鉴定的数千种病毒 circRNA，确定了病毒 circRNA 调节宿主细胞功能的生物学特征和潜在作用。此外，我们开发了一个病毒 circRNA 数据库（http://www.hywanglab.cn/vcRNAdb/），该数据库向所有用户开放，供其搜索、浏览和下载病毒感染时编码的 circRNA 信息。
