Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.
Graduate School of Nanobioscience, Yokohama City University, Yokohama, Kanagawa, Japan.
Methods Mol Biol. 2022;2505:33-43. doi: 10.1007/978-1-0716-2349-7_2.
To understand how the plant regulates metabolism, it is important to determine where metabolites localize in the tissues and cells. Single-cell level omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in Catharanthus roseus stem and leaf tissues.
为了了解植物如何调节新陈代谢，确定代谢物在组织和细胞中的定位是很重要的。近年来，植物中单细胞水平组学方法取得了显著发展，这些数据对于发现参与代谢的酶和转运蛋白的基因具有重要作用。对于代谢组学来说，成像质谱（IMS）是一种绘制组织中分子空间分布的有力工具。在这里，我们描述了用于揭示长春花茎和叶组织中次生代谢物（主要是生物碱）定位的方法。
