从头状夹竹桃叶片外植体诱导不定芽发生和根癌农杆菌介导技术提高转基因植株。

De Novo Shoot Bud Induction from the Catharanthus roseus Leaf Explants and Agrobacterium tumefaciens-Mediated Technique to Raise Transgenic Plants.

机构信息

CSIR-National Chemical Laboratory, Division of Biochemical Sciences, Pune, Maharashtra, India.

Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, Uttar Pradesh, India.

出版信息

Methods Mol Biol. 2022;2505:293-299. doi: 10.1007/978-1-0716-2349-7_21.

DOI:10.1007/978-1-0716-2349-7_21

PMID:35732953

Abstract

The regeneration of a whole plant from a single cell or organ explant was a valuable task for plant biotechnology. However, important medicinal plants such as Catharanthus roseus have shown recalcitrance to regeneration protocols, thus limiting investigations on MIA metabolism and metabolic engineering in this plant system. In this chapter, successful regeneration protocols were detailed for Catharanthus roseus, either by direct shoot bud induction from leaf explants and Agrobacterium-mediated genetic transformation.

摘要

从单个细胞或器官外植体再生整个植物是植物生物技术的一项有价值的任务。然而，长春花等重要药用植物对再生方案表现出抗性，从而限制了对该植物系统中 MIA 代谢和代谢工程的研究。本章详细介绍了长春花的成功再生方案，要么通过叶片外植体的直接芽诱导，要么通过农杆菌介导的遗传转化。