Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen, Copenhagen, Denmark.
Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 2022;2529:407-417. doi: 10.1007/978-1-0716-2481-4_17.
Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.
脉冲稳定同位素标记与氨基酸培养细胞（pSILAC）与质谱分析相结合，是研究组蛋白翻译后修饰（PTMs）传播的有力工具。我们描述了三重 pSILAC 与新生染色质捕获（NCC）标记新复制 DNA 的脉冲追踪标记的结合。该技术跟踪新合成的和再循环的旧组蛋白，从沉积到传递到子细胞，揭示了基于组蛋白的遗传原则。
