Research and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, India.
Department of Biotechnology, University of Madras, Chennai 600025, Tamil Nadu, India.
Biosensors (Basel). 2022 Jun 4;12(6):389. doi: 10.3390/bios12060389.
Salmonellosis caused by sp. has long been reported all over the world. Despite the availability of various diagnostic methods, easy and effective detection systems are still required. This report describes a dialysis membrane electrode interface disc with immobilized specific antibodies to capture antigenic cells. The interaction of a specific antigen with a mouse anti- monoclonal antibody complexed to rabbit anti-mouse secondary antibody conjugated with HRP and the substrate o-aminophenol resulted in a response signal output current measured using two electrode systems (cadmium reference electrode and glassy carbon working electrode) and an agilent HP34401A 6.5 digital multimeter without a potentiostat or applied potential input. A maximum response signal output current was recorded for various concentrations of viz., 3, 30, 300, 3000, 30,000 and 300,000 cells. The biosensor has a detection limit of three cells, which is very sensitive when compared with other detection sensors. Little non-specific response was observed using , , and sp. The maximum response signal output current for a dialysis membrane electrode interface disc was greater than that for gelatin, collagen, and agarose. The device and technique have a range of biological applications. This novel detection system has great potential for future development and application in surveillance for microbial pathogens.
由 sp.引起的沙门氏菌病早已在世界各地被报道。尽管有各种诊断方法，但仍需要易于使用且有效的检测系统。本报告介绍了一种带有固定化特异性抗体的透析膜电极接口盘，用于捕获抗原细胞。特定抗原与与兔抗鼠二级抗体结合的小鼠抗单克隆抗体复合物的相互作用，与 HRP 和基质邻氨基酚偶联，使用两个电极系统（镉参比电极和玻碳工作电极）和安捷伦 HP34401A 6.5 数字多用表（无电位计或外加电位输入）测量得到响应信号输出电流。记录了各种浓度的 sp.（即 3、30、300、3000、30000 和 300000 个细胞）的最大响应信号输出电流。该生物传感器的检测限为三个细胞，与其他检测传感器相比非常灵敏。用 、 和 sp.观察到的非特异性反应很小。对于透析膜电极接口盘，最大响应信号输出电流大于明胶、胶原蛋白和琼脂糖。该设备和技术具有广泛的生物应用。这种新型检测系统具有很大的发展潜力，可用于微生物病原体的监测。
