Guo Kai-Fei, Peng Xiao-Mei, Tu Jie-Yu, Jin Chan, Zhang Wan-Rong, Chen Xi-Zhu, Liu Yong-Jun, Zha Hong-Guang, Shi Wei, Cao Jun
Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Centre for Invasion Biology, Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming 650504, China.
School of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, China.
Insects. 2022 Jun 10;13(6):535. doi: 10.3390/insects13060535.
The guava fruit fly, , is one of the most destructive pests in the genus and detects environmental odorants mainly through antennal olfactory sensilla phenotypes with nanopores. However, it is unclear whether there are naturally occurring abnormal antennal olfactory sensilla phenotypes that affect olfaction. Here, we found that there were abnormal bulges besides nanopores on the surface of trichoid and basiconic olfactory sensilla in the antennal flagellum of long-term laboratory rearing colony (LTC), and that nanopore number in these olfactory sensilla was also remarkably reduced. Notably, the electroantennogram (EAG) responses of LTC insects to methyl eugenol or β-caryophyllene were inhibited, and their behavioral responses elicited by the same odorants were also impaired. These results revealed naturally occurring abnormal antennal olfactory sensilla phenotypes which were involved in olfactory deficit in providing a platform to further study nanopore-targeted pest control technologies in the future.
番石榴实蝇是该属中最具破坏性的害虫之一，主要通过具有纳米孔的触角嗅觉感器表型来检测环境气味。然而，尚不清楚是否存在影响嗅觉的天然异常触角嗅觉感器表型。在这里，我们发现长期实验室饲养群体（LTC）触角鞭节上的毛形和锥形嗅觉感器表面除了纳米孔外还有异常凸起，并且这些嗅觉感器中的纳米孔数量也显著减少。值得注意的是，LTC昆虫对甲基丁香酚或β-石竹烯的触角电图（EAG）反应受到抑制，并且它们由相同气味剂引发的行为反应也受到损害。这些结果揭示了天然存在的异常触角嗅觉感器表型，其与番石榴实蝇的嗅觉缺陷有关，为未来进一步研究以纳米孔为靶点的害虫防治技术提供了一个平台。
