School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Mar Drugs. 2022 May 25;20(6):345. doi: 10.3390/md20060345.
is an excellent source of protein, and in this research, we assessed the antioxidant and emulsifying effects of Chlorella protein hydrolysate (CPH) using neutral proteases and alkaline proteases, as well as the properties of CPH-derived krill oil-in-water (O/W) emulsions. The CPHs exhibited the ability to scavenge several kinds of free radicals, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), O, hydroxyl, and ABTS. Additionally, the CPHs (5 mg/mL) scavenged approximately 100% of the DPPH and ABTS. The CPHs showed similar emulsifying activities to Tween 20 and excellent foaming activities (max FS 74%), which helped to stabilize the krill oil-in-water emulsion. Less than 10 mg/mL CPHs was able to form fresh krill oil-in-water emulsions; moreover, the CPHs (5 mg/mL) in a krill O/W emulsion were homogenous, opaque, and stable for at least 30 days. Based on their inhibitory effects on the peroxide value (POV) and thiobarbituric acid reactive substances (TRABS), the CPHs were found to be able to inhibit lipid oxidation in both emulsifying systems and krill O/W emulsions. Thus, the CPHs could improve superoxide dismutase (SOD) activities by 5- or 10-fold and decrease the high reactive oxygen species (ROS) level caused by the addition of HO in vitro. In conclusion, health-promoting CPHs could be applied in krill oil-in-water emulsions as both emulsifiers and antioxidants, which could help to improve the oxidative and physical stability of emulsions.
是一种极好的蛋白质来源，在这项研究中，我们使用中性蛋白酶和碱性蛋白酶评估了小球藻蛋白水解物（CPH）的抗氧化和乳化作用，以及由 CPH 衍生的磷虾油/水（O/W）乳液的性质。CPH 表现出清除多种自由基的能力，包括 1,1-二苯基-2-苦基肼（DPPH）、O、羟基和 ABTS。此外，CPH（5mg/mL）清除了约 100%的 DPPH 和 ABTS。CPH 表现出与 Tween 20 相似的乳化活性和优异的泡沫活性（最大 FS 74%），有助于稳定磷虾油/O/W 乳液。小于 10mg/mL 的 CPH 能够形成新鲜的磷虾油/O/W 乳液；此外，5mg/mL 的 CPH 在磷虾 O/W 乳液中是均匀的、不透明的，并且在至少 30 天内稳定。根据它们对过氧化物值（POV）和硫代巴比妥酸反应物（TRABS）的抑制作用，CPH 能够抑制乳化系统和磷虾 O/W 乳液中的脂质氧化。因此，CPH 可以将超氧化物歧化酶（SOD）的活性提高 5 倍或 10 倍，并降低体外添加 HO 引起的高活性氧（ROS）水平。总之，具有促进健康作用的 CPH 可以作为乳化剂和抗氧化剂应用于磷虾油/O/W 乳液中，有助于提高乳液的氧化和物理稳定性。
