抗氧化酶系统、氮代谢和渗透调节物质在缓解玉米自交系盐胁迫中的作用及激素调控机制

The Involvement of Antioxidant Enzyme System, Nitrogen Metabolism and Osmoregulatory Substances in Alleviating Salt Stress in Inbred Maize Lines and Hormone Regulation Mechanisms.

作者信息

Wang Mingquan, Gong Shichen, Fu Lixin, Hu Guanghui, Li Guoliang, Hu Shaoxin, Yang Jianfei

机构信息

Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.

出版信息

Plants (Basel). 2022 Jun 10;11(12):1547. doi: 10.3390/plants11121547.

Salt stress inhibited the growth of maize. B46 and NC236 were chosen as materials and NaCl concentrations (0, 55, 110, 165, and 220 mmol L) were set. We found the activities of SOD, POD, CAT, APX, GR, MDHAR, and DHAR decreased under NaCl stress. Compared with NC236, the contents of AsA and GSH, AsA/DHA and GSH/GSSG of B46 decreased. The content of O, HO, MDA, and EL of B46 increased. The contents of NO and NO decreased, while the content of NH increased under high NaCl concentration. The activities of NR and NiR decreased, while the activities of GS and GOGAT increased first and then decreased. For B46 and NC236, the maximum of NADH-GDH and NAD-GDH appeared at 165 and 110 mmol L NaCl concentration, respectively. Compared with B46, and the GOT and GPT activities of NC236 increased first and then decreased. With the increase of NaCl concentration, the contents of proline, soluble protein, and soluble sugar were increased. The Na content of B46 and NC236 increased, and the K content and K/Na decreased. Compared with NC236, B46 had higher IAA content in leaf, higher Z + ZR content in leaf and root, and lower ABA content in leaf and root.

盐胁迫抑制了玉米的生长。选用B46和NC236作为材料，设置NaCl浓度（0、55、110、165和220 mmol/L）。我们发现，在NaCl胁迫下，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）、单脱氢抗坏血酸还原酶（MDHAR）和脱氢抗坏血酸还原酶（DHAR）的活性降低。与NC236相比，B46的抗坏血酸（AsA）和谷胱甘肽（GSH）含量、AsA/脱氢抗坏血酸（DHA）和GSH/氧化型谷胱甘肽（GSSG）降低。B46的超氧阴离子（O₂⁻）、过氧化氢（H₂O₂）、丙二醛（MDA）和电解质渗漏（EL）含量增加。在高NaCl浓度下，一氧化氮（NO）和亚硝酸盐（NO₂⁻）含量降低，而铵（NH₄⁺）含量增加。硝酸还原酶（NR）和亚硝酸还原酶（NiR）的活性降低，而谷氨酰胺合成酶（GS）和谷氨酸合酶（GOGAT）的活性先升高后降低。对于B46和NC236，烟酰胺腺嘌呤二核苷酸（NADH）-谷氨酸脱氢酶（GDH）和烟酰胺腺嘌呤二核苷酸（NAD）-GDH的最大值分别出现在165和110 mmol/L NaCl浓度下。与B46相比，NC236的谷草转氨酶（GOT）和谷丙转氨酶（GPT）活性先升高后降低。随着NaCl浓度的增加，脯氨酸、可溶性蛋白和可溶性糖的含量增加。B46和NC236的钠（Na）含量增加，钾（K）含量和K/Na降低。与NC236相比，B46叶片中的生长素（IAA）含量较高，叶片和根中的玉米素（Z）+玉米素核苷（ZR）含量较高，叶片和根中的脱落酸（ABA）含量较低。