Ali Arlene Asthana, Ahmad Javed, Baig Mohammad Affan, Ahmad Altaf, A Al-Huqail Asma, Qureshi Mohammad Irfan
Department of Biotechnology, Jamia Millia Islamia, New Delhi 110025, India.
Department of Botany, Aligarh Muslim University, Aligarh 202002, India.
Plants (Basel). 2022 Jun 13;11(12):1559. doi: 10.3390/plants11121559.
Forty-day-old (var. Pusa Jai Kisan) plants were exposed to arsenic (As, 250 µM NaHAsO·7HO) stress. The ameliorative role of ferrous sulfate (2 mM, FeSO·7HO, herein FeSO) was evaluated at 7 days after treatment (7 DAT) and 14 DAT. Whereas, As induced high magnitude oxidative stress, FeSO limited it. In general, As decreased the growth and photosynthetic parameters less when in the presence of FeSO. Furthermore, components of the antioxidant system operated in better coordination with FeSO. Contents of non-protein thiols and phytochelatins were higher with the supply of FeSO. Blue-Native polyacrylamide gel electrophoresis revealed an As-induced decrease in almost every multi-protein-pigment complex (MPC), and an increase in PSII subcomplex, LHCII monomers and free proteins. FeSO supplication helped in the retention of a better stoichiometry of light-harvesting complexes and stabilized every MPC, including supra-molecular complexes, PSI/PSII core dimer/ATP Synthase, Cytochrome b6/f dimer and LHCII dimer. FeSO strengthened the plant defence, perhaps by channelizing iron (Fe) and sulfur (S) to biosynthetic and anabolic pathways. Such metabolism could improve levels of antioxidant enzymes, and the contents of glutathione, and phytochelatins. Important key support might be extended to the chloroplast through better supply of Fe-S clusters. Therefore, our results suggest the importance of both iron and sulfur to combat As-induced stress in the Indian mustard plant at biochemical and molecular levels through enhanced antioxidant potential and proteomic adjustments in the photosynthetic apparatus.
对40日龄（品种为Pusa Jai Kisan）的植株施加砷（As，250 μM NaHAsO·7H₂O）胁迫。在处理后7天（7 DAT）和14天（14 DAT）评估硫酸亚铁（2 mM，FeSO·7H₂O，以下简称FeSO）的改善作用。虽然As诱导了高强度的氧化应激，但FeSO限制了这种应激。总体而言，在有FeSO存在的情况下，As对生长和光合参数的降低作用较小。此外，抗氧化系统的各组分与FeSO协同作用更佳。在供应FeSO的情况下，非蛋白硫醇和植物螯合肽的含量更高。蓝色非变性聚丙烯酰胺凝胶电泳显示，As诱导几乎每个多蛋白色素复合体（MPC）减少，而光系统II亚复合体、捕光色素复合体II单体和游离蛋白增加。补充FeSO有助于维持捕光复合体更好的化学计量，并稳定每个MPC，包括超分子复合体、光系统I/光系统II核心二聚体/ATP合酶、细胞色素b6/f二聚体和捕光色素复合体II二聚体。FeSO可能通过将铁（Fe）和硫（S）导向生物合成和合成代谢途径来增强植物防御。这种代谢可以提高抗氧化酶的水平以及谷胱甘肽和植物螯合肽的含量。通过更好地供应铁硫簇，可能会为叶绿体提供重要的关键支持。因此，我们的结果表明，在生化和分子水平上，铁和硫对于通过增强抗氧化潜力和光合机构中的蛋白质组学调整来对抗印度芥菜中As诱导的胁迫都很重要。
