Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, India.
Protoplasma. 2011 Jul;248(3):591-600. doi: 10.1007/s00709-010-0216-7. Epub 2010 Oct 13.
Lycopersicon esculantum sulfate transporter gene (LeST 1.1) encodes a high-affinity sulfate transporter (HAST) located in root epidermis. In this study, the LeST 1.1 gene was constitutively expressed in Indian mustard (Brassica juncea cv. Pusa Jai Kisan). Transgenic as well as untransformed plants were grown in sulfur-insufficient (25 and 50 μM) and sulfur-sufficient (1,000 μM) conditions for 30 days. Two-fold increase was noticed in the sulfate uptake rate of transgenic plants grown in both sulfur-insufficient and -sufficient conditions as compared to untransformed plants. The transgenic B. juncea plants were able to accumulate higher biomass and showed improved sulfur status even in sulfur-insufficient conditions when compared with untransformed plants. Chlorophyll content, ATP sulfurylase activity and protein content were also higher in transgenic plants than untranformed plants under sulfur-insufficient conditions. Our results, thus, clearly indicate that constitutive expression of LeST 1.1 gene in B. juncea had led to enhanced capacity of sulfur uptake and assimilation even in sulfur-insufficient conditions. This approach can also be used in other crops to enhance their sulfate uptake and assimilation potential under S-insufficient conditions.
番茄硫酸盐转运蛋白基因（LeST 1.1）编码一个位于根表皮的高亲和力硫酸盐转运蛋白（HAST）。在本研究中，LeST 1.1 基因在芥菜（芸薹属植物 cv. Pusa Jai Kisan）中组成型表达。在硫不足（25 和 50 μM）和硫充足（1000 μM）条件下，分别对转基因和非转基因植株进行了 30 天的培养。与非转基因植株相比，在硫不足和充足条件下生长的转基因植株的硫酸盐吸收速率增加了两倍。与非转基因植株相比，在硫不足条件下，转基因芥菜植株能够积累更高的生物量，表现出更好的硫素状态。在硫不足条件下，转基因植株的叶绿素含量、ATP 硫酸化酶活性和蛋白质含量也高于非转基因植株。因此，我们的研究结果清楚地表明，在芥菜中组成型表达 LeST 1.1 基因可增强其在硫不足条件下的硫吸收和同化能力。这种方法也可用于其他作物，以提高它们在 S 不足条件下的硫酸盐吸收和同化潜力。
