Use of Gene to Type the Incidence and Distribution of pXFAS_5235 Plasmid-Bearing Strains of subsp. ST1 in Spain.

() is a phytopathogenic bacterium with a repertoire of self-replicating genetic elements, including plasmids, pathogenicity islands, and prophages. These elements provide potential avenues for horizontal gene transfer both within and between species and have the ability to confer new virulence traits, including the ability to colonize new host plants. However, they can also serve as a 'footprint' to type plasmid-bearing strains. Genome sequencing of several strains of subsp. sequence type (ST) 1 from Mallorca Island, Spain, revealed the presence of a 38 kb plasmid (pXFAS_5235). In this study, we developed a PCR-based typing approach using primers targeting the gene to determine the presence of pXFAS_5235 plasmid or other plasmids carrying this gene in a world-wide collection of 65 strains from different subspecies and STs or in 226 plant samples naturally infected by the bacterium obtained from the different outbreaks of in Spain. The gene was amplified only in the plant samples obtained from Mallorca Island infected by subsp. ST1 and from all Spanish strains belonging to this ST. Maximum-likelihood phylogenetic tree of C revealed a close relatedness among Spanish and Californian strains carrying similar plasmids. Our results confirm previous studies, which suggested that a single introduction event of subsp. ST1 occurred in the Balearic Islands. Further studies on the presence and role of plasmids in strains belonging to the same or different subspecies and STs can provide important information in studies of epidemiology, ecology, and evolution of this plant pathogen.