基于同位素标记标签的癌症细胞和组织的多重定量蛋白质组学分析。
Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags.
机构信息
Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada.
National Research Council, Halifax, NS, Canada.
出版信息
Methods Mol Biol. 2022;2508:211-223. doi: 10.1007/978-1-0716-2376-3_16.
Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.
比较多个样本的癌症蛋白质组可以深入了解癌细胞生物学，并可能为特定癌症亚群提供新的治疗选择。在这里，我们描述了一种使用串联质量标签（TMT）技术在单个分析中同时分析多达 18 个样本的方法，为分析大量肿瘤、细胞系及其扰动铺平了道路。我们描述的过程将在 3-4 天内得到可用于深度 LC-MS/MS 分析的样本。
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