Moyanova S, Rousseva S, Dimov S
Methods Find Exp Clin Pharmacol. 1987 Jan;9(1):39-47.
The effect of kainic acid (KA) microinjected into the dorsal raphe nucleus (NRD) of the cat through glass micropipette by means of an air pressure system in doses ranging from 2.3 to 23.5 nmol on the electroencephalographic (EEG)-reactions elicited by repetitive (6-10 Hz) peripheral (somatic and visual) or central electrical stimulation was investigated. A significant facilitation of the driving reaction evoked by rhythmic subcutaneous electrical stimulation of the forepaw was found after KA, while the driving reaction elicited by repetitive photostimulation did not change. The cortical rhythmic reactions to repetitive electrical stimulation of the specific and nonspecific nuclei of the thalamus and of the caudate nucleus (where inhibitory mechanisms are known to be involved) diminished or disappeared when generalized paroxysmal spontaneous activity occurred after kainic acid. The EEG-reaction to stimulation of the mesencephalic reticular formation was significantly facilitated after KA. The repetitive electrical stimulation of the ventral posterolateral nucleus, the centre median and the mesencephalic reticular formation also provoked the appearance of paroxysmal EEG activity or afterdischarge which in some cases developed into epileptic seizures. The data show that kainic acid injected into the dorsal raphe nucleus increases (probably by disinhibition) the excitability level of some forebrain structures connected with the nucleus.
通过气压系统，使用玻璃微电极将不同剂量（2.3至23.5纳摩尔）的海藻酸（KA）微量注射到猫的中缝背核（NRD）中，研究其对由重复性（6 - 10赫兹）外周（躯体和视觉）或中枢电刺激引发的脑电图（EEG）反应的影响。注射KA后，发现前爪有节奏的皮下电刺激诱发的驱动反应明显增强，而重复性光刺激诱发的驱动反应则没有变化。当海藻酸注射后出现全身性阵发性自发活动时，对丘脑特异性和非特异性核以及尾状核（已知其中涉及抑制机制）进行重复性电刺激所引发的皮层节律反应减弱或消失。注射KA后，对中脑网状结构刺激的EEG反应明显增强。对腹后外侧核、中央中核和中脑网状结构进行重复性电刺激也会引发阵发性EEG活动或后放电，在某些情况下会发展为癫痫发作。数据表明，注射到中缝背核的海藻酸可能通过去抑制作用提高了与该核相连的一些前脑结构的兴奋性水平。
