Ye Mengjun, Hou Meng, Peng Qimin, Jia Sheng, Peng Bin, Yin Fangfang, Li Na, Wang Jinquan
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
Animals (Basel). 2022 Jun 17;12(12):1564. doi: 10.3390/ani12121564.
Both the jejunum and colon release cytokines that interact with intestinal microbiota. However, it is largely unclear which cytokines and microbial populations are involved in the homeostasis of the intestinal ecosystem for sheep health. To address this, we collected contents for isolating microbiota and tissues for determining cytokines from the jejunum and colon of 7-month-old Altay sheep. We used the techniques of 16S rRNA sequencing and ELISA to detect microbial population and cytokine level, respectively. Correlations between microbial population and cytokines were analyzed by Spearman correlation coefficient. The correlation analysis revealed higher populations of , and in the colon than in the jejunum, and IL-6 and IL-12 levels were higher in the jejunum than in the colon. Association analysis further revealed a positive association between IL-10 level and both and population in the jejunum. The analysis also revealed positive associations between IL-6 level and and population, IL-10 and , as well as TNF-α and in the colon. These results indicate a potential interaction between the intestinal microbiota and the host immune system that needs to be further clarified for considering dietary formulations to maintain animal health and disease prevention.
空肠和结肠都会释放与肠道微生物群相互作用的细胞因子。然而，目前尚不清楚哪些细胞因子和微生物种群参与了维持绵羊健康的肠道生态系统的稳态。为了解决这个问题，我们收集了7月龄阿勒泰羊的空肠和结肠内容物用于分离微生物群，并收集组织用于测定细胞因子。我们分别使用16S rRNA测序技术和酶联免疫吸附测定法来检测微生物种群和细胞因子水平。通过斯皮尔曼相关系数分析微生物种群与细胞因子之间的相关性。相关性分析显示，结肠中的 、 和 的种群数量高于空肠，空肠中的IL-6和IL-12水平高于结肠。关联分析进一步揭示，空肠中IL-10水平与 和 的种群数量呈正相关。分析还显示，结肠中IL-6水平与 和 的种群数量、IL-10与 以及TNF-α与 呈正相关。这些结果表明肠道微生物群与宿主免疫系统之间存在潜在的相互作用，为了维持动物健康和预防疾病而考虑饮食配方时，这一相互作用需要进一步阐明。
