纽蛋白在非小细胞肺癌中的表达——一项初步研究
Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary Study.
作者信息
Partynska Aleksandra, Gomulkiewicz Agnieszka, Piotrowska Aleksandra, Grzegrzolka Jedrzej, Rzechonek Adam, Ratajczak-Wielgomas Katarzyna, Podhorska-Okolow Marzenna, Dziegiel Piotr
机构信息
Division of Histology and Embryology, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Department of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, Poland.
出版信息
Biomolecules. 2022 Jun 13;12(6):827. doi: 10.3390/biom12060827.
BACKGROUND
The potential involvement of zyxin (ZYX) in carcinogenesis has been investigated in many cancer types. However, there are a limited number of studies on the role of ZYX in the progression of non-small cell lung cancer (NSCLC). Since lung cancer is one of the most frequently diagnosed carcinomas, the aim of our study was to determine the localization and expression levels of ZYX in NSCLC and to correlate the results with the clinicopathological data.
MATERIALS AND METHODS
The expression of ZYX was assessed in NSCLC cases and in cell lines representing this tumor type. Levels of ZYX were determined in the clinical material using immunohistochemistry (IHC) and Western Blot. Real-time PCR was used to assess mRNA levels. The expression of ZYX was also checked in NSCLC cell lines using real-time PCR, Western Blot, and immunofluorescence/immunocytochemistry.
RESULTS
The results showed lower levels of ZYX in NSCLC cells compared with control tissues. This trend was observed at the protein and mRNA levels. The assays on the NSCLC model also demonstrated lower levels of ZYX in cancer cells compared with control cells.
CONCLUSIONS
The decreased expression of ZYX in NSCLC may indicate a suppressor role of this protein in NSCLC.
背景
在许多癌症类型中，已对桩蛋白（ZYX）在致癌过程中的潜在作用进行了研究。然而，关于ZYX在非小细胞肺癌（NSCLC）进展中作用的研究数量有限。由于肺癌是最常被诊断出的癌症之一，我们研究的目的是确定ZYX在NSCLC中的定位和表达水平，并将结果与临床病理数据相关联。
材料与方法
在NSCLC病例以及代表该肿瘤类型的细胞系中评估ZYX的表达。使用免疫组织化学（IHC）和蛋白质印迹法在临床材料中测定ZYX水平。实时聚合酶链反应（PCR）用于评估mRNA水平。还使用实时PCR、蛋白质印迹法以及免疫荧光/免疫细胞化学在NSCLC细胞系中检测ZYX的表达。
结果
结果显示，与对照组织相比，NSCLC细胞中ZYX水平较低。在蛋白质和mRNA水平均观察到这种趋势。对NSCLC模型的检测还表明，与对照细胞相比，癌细胞中ZYX水平较低。
结论
NSCLC中ZYX表达降低可能表明该蛋白在NSCLC中起抑制作用。
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