Rostampour Rezvan, Bahremand Kiana, Mohammadi Hossein, Roghani Seyed Askar, Shakiba Ebrahim, Goodarzi Mohammad Taghi, Asadi Soheila
Department of Clinical Biochemistry Kermanshah University of Medical Sciences Kermanshah Iran.
Nano Drug Delivery Research Center, Health Technology Institute Kermanshah University of Medical Sciences Kermanshah Iran.
Health Sci Rep. 2024 Aug 1;7(8):e2288. doi: 10.1002/hsr2.2288. eCollection 2024 Aug.
Breast cancer (BC) is considered one of the most common malignant tumors leading to death in women, and genetic factors have a crucial role in BC pathogenesis. Zyxin (ZYX) is one of these factors that may be important in p53 level and function. Thus, the present work aimed to investigate the ZYX gene and protein expression in tumor tissue and matched margin tissue and its correlation with the p53 expression.
In a present case-control study, 30 tumors and 30 matched margin tissues were obtained from Iran Tumor Bank/Tehran University of Medical Sciences. Real-time polymerase chain reaction and western blot analysis techniques were applied to evaluate the genes and protein expression, respectively.
The data showed that expression of the ZYX gene in tumor tissues significantly decreased ( = 0.0274) compared to matched margin tissues. In contrast, the p53 gene expression in tumor tissues had no significant difference with matched margin tissues. Additionally, we observed that ZYX and p53 genes expression in tumor tissues of estrogen receptor-positive patients had significant elevation than estrogen receptor-negative patients ( < 0.001, < 0.001, respectively). The data of the western blot analysis technique showed that protein expression of ZYX ( = 0.0024) and P53 protein ( = 0.0218) in tumor tissues was significantly reduced compared to matched margin tissues. Additionally, our analysis showed a direct and significant correlation between the expression of ZYX and p53 proteins ( = 0.7797, = 0.0126) and expression of ZYX and p53 genes (= 0.3079, = 0.0187).
Based on our observation, ZYX might have a tumor suppressor role and is associated with p53.
乳腺癌（BC）被认为是导致女性死亡的最常见恶性肿瘤之一，遗传因素在乳腺癌发病机制中起关键作用。桩蛋白（ZYX）是这些可能对p53水平和功能很重要的因素之一。因此，本研究旨在调查肿瘤组织和配对边缘组织中ZYX基因和蛋白表达及其与p53表达的相关性。
在本病例对照研究中，从伊朗肿瘤库/德黑兰医科大学获得30个肿瘤组织和30个配对边缘组织。分别应用实时聚合酶链反应和蛋白质印迹分析技术评估基因和蛋白表达。
数据显示，与配对边缘组织相比，肿瘤组织中ZYX基因的表达显著降低（=0.0274）。相比之下，肿瘤组织中p53基因表达与配对边缘组织无显著差异。此外，我们观察到雌激素受体阳性患者肿瘤组织中ZYX和p53基因表达比雌激素受体阴性患者显著升高（分别为<0.001，<0.001）。蛋白质印迹分析技术的数据显示，与配对边缘组织相比，肿瘤组织中ZYX蛋白（=0.0024）和P53蛋白（=0.0218）的表达显著降低。此外，我们的分析显示ZYX和p53蛋白的表达之间存在直接且显著的相关性（=0.7797，=0.0126）以及ZYX和p53基因的表达之间存在相关性（=0.3079，=0.0187）。
基于我们的观察，ZYX可能具有肿瘤抑制作用并与p53相关。
