Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou 225001, China.
CAS Engineering Laboratory for Nanozyme, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2022 Jun 9;23(12):6463. doi: 10.3390/ijms23126463.
Aging is a process associated with blood-brain barrier (BBB) damage and the reduction in neurogenesis, and is the greatest known risk factor for neurodegenerative disorders. However, the effects of FeO nanozymes on neurogenesis have rarely been studied. This study examined the effects of FeO nanozymes on neuronal differentiation in the dentate gyrus (DG) and BBB integrity of D-galactose-induced aged mice. Long-term treatment with FeO nanozymes (10 μg/mL diluted in ddHO daily) markedly increased the doublecortin (DCX) immunoreactivity and decreased BBB injury induced by D-galactose treatment. In addition, the decreases in the levels of antioxidant proteins including superoxide dismutase (SOD) and catalase as well as autophagy-related proteins such as Becin-1, LC3II/I, and Atg7 induced by D-galactose treatment were significantly ameliorated by FeO nanozymes in the DG of the mouse hippocampus. Furthermore, FeO nanozyme treatment showed an inhibitory effect against apoptosis in the hippocampus. In conclusion, FeO nanozymes can relieve neuroblast damage and promote neuroblast differentiation in the hippocampal DG by regulating oxidative stress, apoptosis, and autophagy.
衰老与血脑屏障（BBB）损伤和神经发生减少有关，是神经退行性疾病的最大已知风险因素。然而，FeO 纳米酶对神经发生的影响很少被研究。本研究探讨了 FeO 纳米酶对 D-半乳糖诱导衰老小鼠齿状回（DG）神经元分化和 BBB 完整性的影响。长期用 FeO 纳米酶（每天用 ddHO 稀释至 10 μg/mL）处理可显著增加双皮质素（DCX）免疫反应，并减轻 D-半乳糖处理引起的 BBB 损伤。此外，FeO 纳米酶还可显著改善 D-半乳糖处理引起的抗氧化蛋白（包括超氧化物歧化酶（SOD）和过氧化氢酶）和自噬相关蛋白（Becin-1、LC3II/I 和 Atg7）水平降低，在小鼠海马的 DG 中。此外，FeO 纳米酶处理对海马中的细胞凋亡表现出抑制作用。总之，FeO 纳米酶可通过调节氧化应激、细胞凋亡和自噬来减轻海马 DG 中的神经母细胞损伤并促进其分化。
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