Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol Royal Infirmary, Bristol BS2 8HW, UK.
Int J Mol Sci. 2022 Jun 14;23(12):6633. doi: 10.3390/ijms23126633.
Functional endothelial cells (EC) are a critical interface between blood vessels and the thrombogenic flowing blood. Disruption of this layer can lead to early thrombosis, inflammation, vessel restenosis, and, following coronary (CABG) or peripheral (PABG) artery bypass graft surgery, vein graft failure. Blood-derived ECs have shown potential for vascular tissue engineering applications. Here, we show the development and preliminary testing of a method for deriving porcine endothelial-like cells from blood obtained under clinical conditions for use in translational research. The derived cells show cobblestone morphology and expression of EC markers, similar to those seen in isolated porcine aortic ECs (PAEC), and when exposed to increasing shear stress, they remain viable and show mRNA expression of EC markers similar to PAEC. In addition, we confirm the feasibility of seeding endothelial-like cells onto a decellularised human vein scaffold with approximately 90% lumen coverage at lower passages, and show that increasing cell passage results in reduced endothelial coverage.
功能正常的内皮细胞（EC）是血管与富含血小板的血流之间的重要界面。这层结构的破坏可导致早期血栓形成、炎症、血管再狭窄，并且在冠状动脉（CABG）或外周（PABG）动脉旁路移植手术后，静脉移植物也会失效。血液来源的 EC 已显示出在血管组织工程应用中的潜力。在这里，我们展示了一种从临床条件下获得的血液中提取猪内皮样细胞的方法的开发和初步测试，该方法可用于转化研究。所得到的细胞表现出鹅卵石形态和 EC 标志物的表达，与从分离的猪主动脉 EC（PAEC）中观察到的相似，并且当暴露于逐渐增加的切应力时，它们仍然保持存活并表现出与 PAEC 相似的 EC 标志物的 mRNA 表达。此外，我们还证实了将内皮样细胞接种到脱细胞化的人静脉支架上的可行性，在较低传代时可实现约 90%的管腔覆盖率，并且表明细胞传代增加会导致内皮覆盖率降低。
