Ardila Diana Catalina, Liou Jr-Jiun, Maestas David, Slepian Marvin J, Badowski Michael, Wagner William R., Harris David, Vande Geest Jonathan P
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA.
J Clin Med. 2019 Feb 4;8(2):185. doi: 10.3390/jcm8020185.
Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.
组织工程作为一种开发用于旁路手术的小直径组织工程血管移植物的替代方法而受到关注，这是治疗冠心病的一种选择。为了促进移植物管腔内健康内皮细胞单层的形成，开发了聚己内酯/明胶/纤维蛋白原支架，并通过热成型以及用IV型胶原和纤连蛋白包被对其表面进行修饰。将人脐带血来源的内皮细胞（hCB-ECs）接种到支架上，并以人脐静脉内皮细胞作为对照，在静态条件下评估健康内皮细胞层的重要特征。我们发现，经过热成型和包被的聚己内酯/明胶/纤维蛋白原支架最适合内皮细胞生长。hCB-ECs能够增殖、产生内皮型一氧化氮合酶、对白细胞介素1β作出反应并减少血小板沉积。
