Rogers M V, McLaren D J
Mol Biochem Parasitol. 1987 Jan 15;22(2-3):273-88. doi: 10.1016/0166-6851(87)90058-2.
Lipids extracted from whole worm homogenates and tegumental outer membranes of guinea pig-derived 5-day, 2-, 3- and 6-week old schistosomes have been analysed by thin layer chromatography. Six-week hamster-derived parasites have been studied for comparative purposes. All homogenates contained neutral lipids, cholesterol and several phospholipids; phosphatidyl choline and phosphatidyl ethanolamine were major components. Phospholipid (P3) was absent from homogenates of 5-day worms but was present in older parasites. A single phospholipid (P4) which co-chromatographed with phosphatidyl glycerol was unique to hamster-derived parasites. One glycolipid (G1) was ubiquitous to all homogenates and co-chromatographed with the monogalactosyl ceramide marker. A second sugar-containing lipid (G2) was unique to 3-week worm homogenates, and was highly polar. It was resolved beneath the trigalactosyl ceramide marker. Tegumental membranes isolated from 6-week adults contained at least five glycolipids, four of which were also highly polar. Cholesterol and two dominant phospholipids occurred in the membranes of 2-, 3-, and 6-week worms. One phospholipid co-chromatographed with phosphatidyl choline; the other had an Rf value (relative band speed) equivalent to phosphatidyl ethanolamine. Membranes from liver stage parasites contained a further phospholipid which cochromatographed with sphingomyelin, and three additional, phosphate-staining lipids (P1, P3 and P6). Five sugar-containing lipids occurred in adult membranes only; four were highly polar, being resolved near the origin. Similar components were identified in extracts of host erythrocytes. The remaining membrane lipid appeared homologous to G1 identified in the whole worm homogenates. Important changes in lipid composition thus occur during schistosome growth and maturation in guinea pigs; moreover, worms derived from different rodents express different lipids.
通过薄层色谱法分析了从豚鼠来源的5日龄、2周龄、3周龄和6周龄血吸虫的全虫匀浆以及皮层外膜中提取的脂质。为了进行比较，还研究了6周龄仓鼠来源的寄生虫。所有匀浆都含有中性脂质、胆固醇和几种磷脂；磷脂酰胆碱和磷脂酰乙醇胺是主要成分。5日龄虫体的匀浆中不存在磷脂（P3），但在较老的寄生虫中存在。一种与磷脂酰甘油共色谱的单一磷脂（P4）是仓鼠来源寄生虫所特有的。一种糖脂（G1）在所有匀浆中都存在，并与单半乳糖基神经酰胺标记物共色谱。第二种含糖脂质（G2）是3周龄虫体匀浆所特有的，且极性很高。它在三半乳糖基神经酰胺标记物下方分离出来。从6周龄成虫分离的皮层膜含有至少五种糖脂，其中四种也极性很高。胆固醇和两种主要磷脂存在于2周龄、3周龄和6周龄虫体的膜中。一种磷脂与磷脂酰胆碱共色谱；另一种的Rf值（相对条带速度）与磷脂酰乙醇胺相当。肝期寄生虫的膜含有另一种与鞘磷脂共色谱的磷脂，以及另外三种磷酸染色脂质（P1、P3和P6）。仅在成虫膜中发现了五种含糖脂质；四种极性很高，在原点附近分离出来。在宿主红细胞提取物中也鉴定出了类似的成分。其余的膜脂质似乎与全虫匀浆中鉴定出的G1同源。因此，在豚鼠体内血吸虫的生长和成熟过程中脂质组成发生了重要变化；此外，来自不同啮齿动物的虫体表达不同的脂质。
