Matchanov Alimjon D, Esanov Rakhmat S, Renkawitz Tobias, Soliev Azamjon B, Kunisch Elke, Gonzalo de Juan Isabel, Westhauser Fabian, Tulyaganov Dilshat U
Institute of bioorganic Chemistry, Mirzo Ulugbek, 83, Tashkent 100170, Uzbekistan.
Department of Chemistry, National University of Uzbekistan, University Street 4, Tashkent 100174, Uzbekistan.
Materials (Basel). 2022 Jun 13;15(12):4197. doi: 10.3390/ma15124197.
Medical nutrients obtained from plants have been used in traditional medicine since ancient times, owning to the protective and therapeutic properties of plant extracts and products. Glycyrrhizic acid is one of those that, apart from its therapeutic effect, may contribute to stronger bones, inhibiting bone resorption and improving the bone structure and biomechanical strength. In the present study, we investigated the effect of a bioactive glass (BG) addition to the structure-property relationships of supramolecular assemblies formed by glycyrrhizic acid (GA) and its monoammonium salt (MSGA). FTIR spectra of supramolecular assemblies evidenced an interaction between BG components and hydroxyl groups of MSGA and GA. Moreover, it was revealed that BG components may interact and bond to the carboxyl groups of MSGA. In order to assess their biological effects, BG, MSGA, and their supramolecular assemblies were introduced to a culture of human bone-marrow-derived mesenchymal stromal cells (BMSCs). Both the BG and MSGA had positive influence on BMSC growth, viability, and osteogenic differentiation-these positive effects were most pronounced when BG1d-BG and MSGA were introduced together into cell culture in the form of MSGA:BG assemblies. In conclusion, MSGA:BG assemblies revealed a promising potential as a candidate material intended for application in bone defect reconstruction and bone tissue engineering approaches.
自古以来，从植物中获取的医用营养物质就被用于传统医学，这归因于植物提取物和产品的保护及治疗特性。甘草酸就是其中之一，除了具有治疗作用外，它可能有助于增强骨骼，抑制骨吸收并改善骨骼结构和生物力学强度。在本研究中，我们研究了添加生物活性玻璃（BG）对由甘草酸（GA）及其单铵盐（MSGA）形成的超分子组装体的结构-性能关系的影响。超分子组装体的傅里叶变换红外光谱（FTIR）表明BG成分与MSGA和GA的羟基之间存在相互作用。此外，还发现BG成分可能与MSGA的羧基相互作用并结合。为了评估它们的生物学效应，将BG、MSGA及其超分子组装体引入人骨髓间充质基质细胞（BMSC）培养物中。BG和MSGA对BMSC的生长、活力和成骨分化均有积极影响——当以MSGA:BG组装体的形式将BG1d-BG和MSGA一起引入细胞培养时，这些积极作用最为明显。总之，MSGA:BG组装体作为一种有望应用于骨缺损修复和骨组织工程方法的候选材料，显示出了巨大潜力。
