Tsuka Takeshi, Kumashiro Soma, Kihara Tsubasa, Iida Toshiko
Clinical Veterinary Sciences, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
Microorganisms. 2022 May 31;10(6):1138. doi: 10.3390/microorganisms10061138.
We investigated the correlation between the polymerase chain reaction (PCR) identification of six virulence genes associated with siderophore activation and the iron-uptake system (iron-acquisition genes; A, B, A, S, , and ) in mastitis-associated (). The growth of 37 isolates from the milk of cows with mild mastitis reared on Japanese dairy farms between October 2012 and December 2014 was examined by incubation in an iron-deficient medium. B-, A-, or S-positive isolates grew significantly better than B-, A-, or S-negative isolates after incubating in an iron-deficient medium for three days. Interestingly, the growth of isolates with 0 and ≥4 PCR-positive iron-acquisition genes in the iron-deficient medium were significantly different by day 2, while isolates with 2, 3, and ≥4 PCR-positive iron-acquisition genes grew significantly better than those with no PCR-positive iron-acquisition genes by day 3. Based on the correlation between the results of PCR and iron-deficient incubation tests, iron-deficient incubation for three days can be used to estimate the presence or absence of iron-acquisition genes in mastitis-associated .
我们研究了与铁载体激活相关的六个毒力基因的聚合酶链反应（PCR）鉴定与乳腺炎相关的金黄色葡萄球菌中铁摄取系统（铁获取基因；A、B、A、S、 和 ）之间的相关性。对2012年10月至2014年12月期间在日本奶牛场饲养的患有轻度乳腺炎的奶牛乳汁中分离出的37株金黄色葡萄球菌，通过在缺铁培养基中培养来检测其生长情况。在缺铁培养基中培养三天后，B、A或S阳性分离株的生长明显优于B、A或S阴性分离株。有趣的是，在缺铁培养基中，0个和≥4个PCR阳性铁获取基因的分离株在第2天生长情况有显著差异，而在第3天，具有2个、3个和≥4个PCR阳性铁获取基因的分离株比没有PCR阳性铁获取基因的分离株生长得明显更好。基于PCR结果与缺铁培养试验结果之间的相关性，缺铁培养三天可用于估计乳腺炎相关金黄色葡萄球菌中铁获取基因的有无。
