Kimmel C B, Warga R M
Nature. 1987;327(6119):234-7. doi: 10.1038/327234a0.
Cell lineage may contribute to determining the numbers, positions and types of cells formed during embryogenesis. In vitro clonal analyses show that vertebrate cells can autonomously maintain lineage commitments to single fates and that terminal development may include an invariant sequence of cell divisions. In addition, in vivo studies with Xenopus led to the proposal that clonal restrictions to spatial 'compartmental' domains arise during early development, analogous to what is observed in insects. In the zebrafish, individual gastrula cells generate clones of progeny that are confined within single tissues, but spatial restrictions have not been described. We now have examined the in vivo terminal cell lineages of zebrafish axial muscles. We obtained no evidence either for strict developmental regulation of division pattern or for spatial compartmentation within muscle lineages.
细胞谱系可能有助于确定胚胎发育过程中形成的细胞数量、位置和类型。体外克隆分析表明，脊椎动物细胞能够自主维持对单一命运的谱系承诺，并且终末发育可能包括细胞分裂的固定序列。此外，对非洲爪蟾的体内研究提出，在早期发育过程中会出现对空间“分区”区域的克隆限制，这与在昆虫中观察到的情况类似。在斑马鱼中，单个原肠胚细胞产生的后代克隆局限于单一组织内，但尚未描述空间限制情况。我们现在研究了斑马鱼轴向肌肉的体内终末细胞谱系。我们没有获得证据证明肌肉谱系内存在严格的分裂模式发育调控或空间分隔。
