Bedwani J R, Mehta A
Neurochem Res. 1987 Jan;12(1):49-52. doi: 10.1007/BF00971363.
The release of [3H]gamma-aminobutyric acid (GABA) from pre-loaded slices of rat cerebral cortex was investigated in the presence and absence of the GABA-transaminase inhibitors gabaculine and gamma-vinyl GABA. In the experiments carried out without an inhibitor, an ion-exchange column chromatographic technique was used to separate [3H]GABA from tritiated metabolites released with it into the superfusate. The presence of gabaculine (5 microM) substantially reduced the Ca2+-dependence of the release of [3H]GABA evoked by a 4 min 30 mM K+ pulse, whereas this was not appreciably reduced by the presence of gamma-vinyl GABA (2 mM or 10 mM). Nevertheless, the characteristics of [3H]GABA release were not identical in the presence and absence of either inhibitor.
在存在和不存在γ-氨基丁酸转氨酶抑制剂加巴喷丁和γ-乙烯基γ-氨基丁酸（GABA）的情况下，研究了预先加载的大鼠大脑皮层切片中[3H]γ-氨基丁酸（GABA）的释放情况。在没有抑制剂的实验中，使用离子交换柱色谱技术将[3H]GABA与其释放到超滤液中的氚化代谢物分离。加巴喷丁（5 microM）的存在显著降低了由4分钟30 mM K+脉冲诱发的[3H]GABA释放对Ca2+的依赖性，而γ-乙烯基GABA（2 mM或10 mM）的存在对此没有明显降低作用。然而，在存在或不存在任何一种抑制剂的情况下，[3H]GABA释放的特征并不相同。
