Gardner C R, Richards M H
J Neurochem. 1981 Apr;36(4):1590-3. doi: 10.1111/j.1471-4159.1981.tb00604.x.
Most studies on gamma-aminobutyric acid (GABA) release from nervous tissue have been conducted using radiolabelled GABA in the presence of aminooxyacetic acid (AOAA) to inhibit GABA: 2-oxoglutarate aminotransferase (GABA-T) to prevent conversion of labelled GABA to labelled catabolites. Here we present data showing that even in the presence of 10 microM-AOAA the spontaneous release of tritium from rat cortical synaptosomes prelabelled with 2,3-[3H]GABA is mainly in the form of tritiated water but that the increase in tritium release in the presence of unlabelled GABA or high potassium-ion concentrations is in the form of authentic [3H]GABA. Interpretation of results should take these facts into account.
大多数关于从神经组织释放γ-氨基丁酸（GABA）的研究都是在氨基氧基乙酸（AOAA）存在的情况下，使用放射性标记的GABA进行的，以抑制GABA：2-氧代戊二酸氨基转移酶（GABA-T），防止标记的GABA转化为标记的分解代谢物。在此，我们展示的数据表明，即使在存在10微摩尔AOAA的情况下，预先用2,3-[3H]GABA标记的大鼠皮质突触体中氚的自发释放主要是以氚化水的形式，但在未标记的GABA或高钾离子浓度存在的情况下，氚释放的增加是以真实的[3H]GABA的形式。结果的解释应考虑到这些事实。
