Sketelj J, Crne N, Brzin M
Neurochem Res. 1987 Feb;12(2):159-65. doi: 10.1007/BF00979532.
Molecular forms and histochemical localization of acetylcholinesterase and nonspecific cholinesterase were analysed in muscle regenerates obtained from rat EDL and soleus muscles after ischaemic-toxic degeneration and irreversible inhibition of preexistent enzymes. Regenerating myotubes and myofibres produce the 16S AChE form in the absence of innervation. The 10S AChE form prevails over 4S form with maturation into striated fibres. Although the patterns of AChE molecular forms in normal EDL and soleus muscles differ significantly no such differences were observed in noninnervated regenerates from both muscles. Two types of focal accumulation of AChE appear on the sarcolemma of regenerating muscles: first, in places of former motor endplates and, second, in extra-junctional regions. The 4S form of nonspecific cholinesterase is prevailing in regenerating myotubes whereas its asymmetric forms or focal accumulations could not be identified reliably. The satellite cells which survive after muscle degeneration probably originate from some type of late myoblasts and transmit the information concerning the ability to synthesize the asymmetric AChE forms and to focally accumulate AChE to regenerating muscle cells. Synaptic basal lamina from former motor endplates may locally induce AChE accumulations in regenerating muscles.
在缺血性毒性变性以及对先前存在的酶进行不可逆抑制后，从大鼠趾长伸肌（EDL）和比目鱼肌获取肌肉再生组织，分析乙酰胆碱酯酶和非特异性胆碱酯酶的分子形式及组织化学定位。在无神经支配的情况下，再生的肌管和肌纤维产生16S乙酰胆碱酯酶形式。随着成熟为横纹肌纤维，10S乙酰胆碱酯酶形式比4S形式更为普遍。尽管正常EDL肌和比目鱼肌中乙酰胆碱酯酶分子形式的模式有显著差异，但在这两种肌肉的无神经支配再生组织中未观察到此类差异。再生肌肉的肌膜上出现两种类型的乙酰胆碱酯酶局灶性积聚：第一，在先前运动终板的位置；第二，在结外区域。非特异性胆碱酯酶的4S形式在再生肌管中占主导，而其不对称形式或局灶性积聚无法可靠识别。肌肉变性后存活的卫星细胞可能起源于某种晚期成肌细胞，并将有关合成不对称乙酰胆碱酯酶形式以及在再生肌细胞中局灶性积聚乙酰胆碱酯酶能力的信息传递给它们。来自先前运动终板的突触基膜可能在再生肌肉中局部诱导乙酰胆碱酯酶积聚。
