Ottlecz A, Telegdy G
Neuropeptides. 1987 Feb-Mar;9(2):161-7. doi: 10.1016/0143-4179(87)90054-0.
The presence of FMRFamide (Phe-Met-Arg-Phe-amide)-like immunoreactivity in neuronal elements in the hypothalamus suggested a role for this in the hypothalamic control of the anterior pituitary function. We report here the action of FMRFamide on growth hormone release following intracerebroventricular administration to rats. The injection of 200 ng (313.8 picomoles) of FMRFamide (in 2 ul) produced a significantly increased plasma GH 15 min after injection. The GH-increasing effect of 400-800 ng (627-1255 picomoles) of FMRFamide was already developed after 5 min and lasted up to 30 min. No change was detected in the plasma FSH, LH and prolactin levels at any time during the experimental period. The intravenous administration of 10, 30 or 100 ug of FMRFamide had no effect on the plasma GH level. We conclude that FMRFamide can act at low doses to increase GH release through the inhibition of somatostatin release or the stimulation of GRF. We could not exclude a direct site of action in the pituitaries.
下丘脑神经元中存在类似FMRF酰胺（苯丙氨酸-甲硫氨酸-精氨酸-苯丙氨酸酰胺）的免疫反应性，这表明其在垂体前叶功能的下丘脑控制中发挥作用。我们在此报告向大鼠脑室内注射FMRF酰胺后对生长激素释放的作用。注射200 ng（313.8皮摩尔）FMRF酰胺（溶于2 μl）后15分钟，血浆生长激素显著增加。400 - 800 ng（627 - 1255皮摩尔）FMRF酰胺的生长激素增加作用在5分钟后即已出现，并持续长达30分钟。在实验期间的任何时候，血浆促卵泡激素、促黄体生成素和催乳素水平均未检测到变化。静脉注射10、30或100 μg FMRF酰胺对血浆生长激素水平无影响。我们得出结论，FMRF酰胺可在低剂量下起作用，通过抑制生长抑素释放或刺激生长激素释放因子来增加生长激素释放。我们不能排除其在垂体中有直接作用位点。
