Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Ocean College, Agricultural University of Hebei, Qinhuangdao 066000, China.
Viruses. 2022 Jun 2;14(6):1216. doi: 10.3390/v14061216.
A novel nidovirus, CSBV Bces-Po19, was isolated from the marine fish, Japanese flounder (). The viral genome was 26,597 nucleotides long and shared 98.62% nucleotide identity with CSBV WHQSR4345. PacBio Sequel and Illumina sequencing were used to perform full-length transcriptome sequencing on CSBV Bces-Po19-sensitive (S) and -resistant (R) Japanese flounder. The results of negative staining revealed bacilliform and spherical virions. There were in total 1444 different genes between CSBV Bces-Po19 S and R groups, with 935 being up-regulated and 513 being down-regulated. Metabolism-, immune-, and RNA-related pathways were significantly enriched. Furthermore, CSBV Bces-Po19 infection induced alternative splicing (AS) events in Japanese flounder; the S group had a higher numbers of AS events (12,352) than the R group (11,452). The number of long non-coding RNA (lncRNA) in the S group, on the other hand, was significantly lower than in the R group. In addition to providing valuable information that sheds more light on CSBV Bces-Po19 infection, these research findings provide further clues for CSBV Bces-Po19 prevention and treatment.
一种新型尼多病毒，CSBV Bces-Po19，从海洋鱼类牙鲆（）中分离出来。该病毒基因组全长 26597 个核苷酸，与 CSBV WHQSR4345 的核苷酸序列同源性为 98.62%。PacBio Sequel 和 Illumina 测序技术被用于对 CSBV Bces-Po19 敏感（S）和抗性（R）牙鲆进行全长转录组测序。负染结果显示出杆状和球形病毒粒子。CSBV Bces-Po19 S 和 R 组之间共有 1444 个不同的基因，其中 935 个上调，513 个下调。代谢、免疫和 RNA 相关途径显著富集。此外，CSBV Bces-Po19 感染诱导牙鲆发生可变剪接（AS）事件；S 组的 AS 事件（12352 个）比 R 组（11452 个）多。另一方面，S 组的长非编码 RNA（lncRNA）数量明显低于 R 组。这些研究结果不仅为 CSBV Bces-Po19 感染提供了有价值的信息，也为 CSBV Bces-Po19 的预防和治疗提供了进一步的线索。
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