Rizzo Roberta, Bortolotti Daria, Morandi Luca, Rizzo Sabrina, Schiuma Giovanna, Beltrami Silvia, Papi Alberto, Contoli Marco
Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Italy.
Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Italy.
Int J Infect Dis. 2022 Sep;122:412-414. doi: 10.1016/j.ijid.2022.06.020. Epub 2022 Jun 21.
Vaccines against SARS-CoV-2 ameliorate infection and adverse outcomes from SARS-CoV-2. Elicitation of high affinity and durable protective antibody responses is a hallmark of a successful humoral immune response to vaccination. To assess the relevance of serum levels of SARS-CoV-2 specific antibodies and to further characterize the immune response to SARS-CoV-2 vaccines, we report i) the levels of spike-binding and neutralizing antibodies to SARS-COV-2 in the sera of 30 healthy volunteers at nine months after the second vaccination dose of mRNA vaccine and one month after the booster dose; ii) the levels of IFN-γ production by blood T cells exposed to SARS-CoV-2 spike antigen (Wuhan, Alpha B.1.1.7, Delta B.1.617.2, and Omicron B1.1.529 variants); and iii) the specific phenotype of T cells related with exposure to SARS-CoV-2 spike antigen. We observed that the booster dose induced increased humoral and adaptive immune responses and led to early activation of the memory CD8+ T subset.
针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的疫苗可改善SARS-CoV-2感染及不良后果。引发高亲和力和持久的保护性抗体反应是疫苗接种成功的体液免疫反应的一个标志。为了评估SARS-CoV-2特异性抗体血清水平的相关性，并进一步表征对SARS-CoV-2疫苗的免疫反应，我们报告了：i）30名健康志愿者在接种第二剂mRNA疫苗九个月后和接种加强针一个月后的血清中针对SARS-CoV-2的刺突结合抗体和中和抗体水平；ii）暴露于SARS-CoV-2刺突抗原（武汉株、阿尔法B.1.1.7、德尔塔B.1.617.2和奥密克戎B1.1.529变体）的血液T细胞产生干扰素-γ的水平；以及iii）与暴露于SARS-CoV-2刺突抗原相关的T细胞的特定表型。我们观察到，加强针诱导了体液和适应性免疫反应增强，并导致记忆性CD8+T亚群的早期激活。
