São Paulo State University (UNESP), Institute of Chemistry, Department of Analytical, Physicochemical and Inorganic Chemistry, National Institute of Alternative Technologies for Detection, Toxicological Assessment and Removal of Micropollutants and Radioactives (INCT-DATREM), Rua Professor Francisco Degni, 55, Araraquara 14800-060, São Paulo, Brazil; São Paulo State University (UNESP), Institute of Chemistry, Department of Biochemistry and Organic Chemistry, Rua Professor Francisco Degni, 55, Araraquara 14800-060, São Paulo, Brazil.
São Paulo State University (UNESP), Institute of Chemistry, Department of Analytical, Physicochemical and Inorganic Chemistry, National Institute of Alternative Technologies for Detection, Toxicological Assessment and Removal of Micropollutants and Radioactives (INCT-DATREM), Rua Professor Francisco Degni, 55, Araraquara 14800-060, São Paulo, Brazil.
Bioelectrochemistry. 2022 Oct;147:108177. doi: 10.1016/j.bioelechem.2022.108177. Epub 2022 Jun 13.
The present work describes an easy way to prepare a Chloroplast/PDA@WO biohybrid platform based on the deposition of chloroplasts on WO substrate previously modified with polydopamine (PDA) film as anchoring agent. The use of PDA as an immobilization matrix for chloroplasts, and also as an electron mediator under LED irradiation, resulted in enhanced photocurrents. The use of the chloroplasts amplified the photocurrent, when compared to the bare substrate (WO). The best electrode performance was obtained using high intensity LED irradiation at 395 nm, for the electrode exposed for 10 min to 150 μg mL of intact chloroplasts. Amperometric curves obtained by on/off cycles using an applied potential of +0.50 V, in PBS electrolyte (pH 7.0), showed that the presence of 0.2 × 10 mol L of simazine caused an approximately 50% decrease of the photobiocurrent. Preliminary studies indicated that the synthesized platform based on intact chloroplasts is a good strategy for studying the behavior of photosynthetic entities, using an LED light-responsive WO semiconductor substrate. This work contributes to the understanding of photobiocatalysts that emerge as a new class of materials with sophisticated and intricate structures. These are promising materials with remarkably improved quantum efficiency with potential applications in photobioelectrocatalysis.
本工作描述了一种基于叶绿体/PDA@WO 生物杂化平台的简便制备方法，该平台基于先前用聚多巴胺（PDA）薄膜修饰的 WO 基底上沉积叶绿体作为附着剂。使用 PDA 作为叶绿体的固定基质，以及在 LED 照射下作为电子介体，导致光电流增强。与裸基底（WO）相比，使用叶绿体放大了光电流。使用高强度 LED 在 395nm 下照射 10 分钟，对于暴露于 150μg mL 完整叶绿体的电极，获得了最佳的电极性能。在 PBS 电解质（pH 7.0）中使用+0.50V 的施加电位进行的 on/off 循环获得的安培曲线表明，存在 0.2×10-6mol L 的西玛津导致光生物电流降低约 50%。初步研究表明，基于完整叶绿体的合成平台是研究使用 LED 光响应 WO 半导体基底的光合作用实体行为的一种很好的策略。这项工作有助于理解作为具有复杂结构的新型材料的光生物催化剂。这些是很有前途的材料，具有显著提高的量子效率，在光生物电化学催化中有潜在的应用。
