Department of Reproduction, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey.
Department of Histology and Embiyology, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey.
Anim Reprod Sci. 2022 Aug;243:107026. doi: 10.1016/j.anireprosci.2022.107026. Epub 2022 Jun 15.
The study aimed to investigate the expression and cellular localization of two critical non-nuclear progesterone receptors, including membrane-associated-progesterone-receptor-component-1 (PGRMC1) and progestin and adipoQ receptor family member 7 (PAQR7) throughout the estrous cycle and early pregnancy in ovine corpus luteum (CL). Ewes were randomly grouped into cyclic (C, n = 4 per group) or pregnant (P, n = 4 per group) groups. Following slaughtering, the CL was obtained from both cyclic and pregnant ewes on days 12 (C12 and P12), 16 (C16 and P16), and 22 (C22 and P22). Western blotting and RT-qPCR were utilized to assess the expression levels of PGRMC1 and PAQR7, whereas immunohistochemistry was performed to determine the localization of PGRMC1 and PAQR7 in CL. Data were evaluated by one-way ANOVA, and the P < 0.05 was considered a significant difference. PGRMC1 was shown to be expressed in both small and large luteal cells and endothelial cells in CL, while PAQR7 expression was only found in small and large luteal cells. Compared to cycle days, pregnancy increased the expression of PGRMC1. PAQR7 did not differ during early pregnancy but reduced during the functional luteolysis stage (C16). mRNA and protein expression patterns for PGRMC1 and PAQR7 were similar on the studied days. This is the first study that demonstrates the expression and cellular localization of PGRMC1 and PAQR7 in ovine CL. We suggest that these receptors could execute a significant role in the ovine CL life span in both cyclic changes and the establishment of pregnancy.
本研究旨在探究两种关键的非核孕激素受体——膜相关孕激素受体成分 1（PGRMC1）和孕激素及脂联素 Q 受体家族成员 7（PAQR7）——在绵羊黄体（CL）发情周期和早期妊娠过程中的表达和细胞定位。将母羊随机分为发情周期（C，每组 4 只）或妊娠（P，每组 4 只）组。屠宰后，于发情周期第 12 天（C12 和 P12）、第 16 天（C16 和 P16）和第 22 天（C22 和 P22）分别从发情周期和妊娠母羊的 CL 中获取样本。利用 Western blot 和 RT-qPCR 评估 PGRMC1 和 PAQR7 的表达水平，采用免疫组织化学方法确定 PGRMC1 和 PAQR7 在 CL 中的定位。数据采用单因素方差分析进行评估，P<0.05 为差异有统计学意义。结果表明，PGRMC1 在 CL 的小黄体细胞和大黄体细胞以及内皮细胞中均有表达，而 PAQR7 仅在小黄体细胞和大黄体细胞中表达。与发情周期相比，妊娠增加了 PGRMC1 的表达。PAQR7 在妊娠早期没有差异，但在功能黄体溶解阶段（C16）减少。在研究的天数中，PGRMC1 和 PAQR7 的 mRNA 和蛋白表达模式相似。这是首次研究表明 PGRMC1 和 PAQR7 在绵羊 CL 中的表达和细胞定位。我们认为，这些受体在绵羊 CL 的发情周期变化和妊娠建立过程中可能发挥重要作用。
