Kowalik Magdalena K, Kotwica Jan
Institute of Animal Reproduction and Food Research, PAS, Tuwima St 10, Olsztyn 10-747, Poland.
Reprod Biol. 2008 Nov;8(3):291-7. doi: 10.1016/s1642-431x(12)60019-9.
The aim of the study was to investigate progesterone receptor membrane component 1 (PGRMC1) mRNA expression in bovine corpus luteum (CL) obtained from heifers or non-pregnant cows on the following days of the estrous cycle: 1-5, 6-10, 11-16 and 17-21 (n=4/each time period). The expression of PGRMC1 mRNA, analyzed by semiquantitative RT-PCR, was the highest on days 6-10 (p<0.01) and then it declined (p<0.05). The lowest expression was found on days 1-5 (p<0.05). A significant correlation (p<0.05) was also observed between luteal progesterone (P(4)) concentration and PGRMC1 mRNA expression. These data indicate that PGRMC1 mRNA is expressed in bovine CL and this expression varies throughout the luteal phase. It is assumed that PGRMC1 may be involved in a non-genomic effect of P(4) on luteal cells.
本研究的目的是调查在发情周期的以下几天，从青年母牛或未怀孕母牛获得的牛黄体（CL）中孕激素受体膜成分1（PGRMC1）mRNA的表达情况：第1 - 5天、第6 - 10天、第11 - 16天和第17 - 21天（每个时间段n = 4）。通过半定量RT-PCR分析，PGRMC1 mRNA的表达在第6 - 10天最高（p<0.01），然后下降（p<0.05）。在第1 - 5天表达最低（p<0.05）。黄体孕酮（P(4)）浓度与PGRMC1 mRNA表达之间也观察到显著相关性（p<0.05）。这些数据表明PGRMC1 mRNA在牛黄体中表达，且这种表达在黄体期会发生变化。推测PGRMC1可能参与P(4)对黄体细胞的非基因组效应。
