Highly efficient red-emitting carbon dots as a "turn-on" temperature probe in living cells.

Nanothermometers, which can precisely detect the intracellular temperature changes, have great potential to solve questions concerning the cellular processes. Thus, the temperature sensors that provide fluorescent "turn-on" signals in the biological transparency window are of highly desirable. To meet these criteria, this work reported a new "turn-on" carbon dot (CD)-based fluorescent nanothermometry device for sensing temperature in living cells. The CDs that emit bright red fluorescence (R-CDs; λ = 610 nm in water) were synthesized with o-phenylenediamine as carbon precursor via a facile solvothermal method. The R-CDs in water were almost nonfluorescent at 15 °C. As the temperature increased, the fluorescence intensity of R-CDs exhibited a gradual increase and the final enhancement factor was greater than 21-fold. The fluorescence intensity exhibited a linear response to temperature and a high-sensitive variation of ≈13.3 % °C was detected within a broad temperature range of 28-60 °C. Moreover, the R-CD thermal sensors also exhibited high storage stability, excellent response reversibility and superior photo- and thermo-stability. Due to its good biocompatibility and "intelligent" response to external temperature, the nanothermometer could be applied for sensing temperature changes in biological media.