利用 UniSpray 离子源对尿液中的三种抗肿瘤药物进行定量分析。

Quantification of three antineoplastic agents in urine using the UniSpray ionisation source.

机构信息

Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.

Unit Environmental Hygiene and Human Biological Monitoring, Department of Health Protection, Laboratoire National de Santé (LNS), Dudelange, Luxembourg.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Aug 1;1205:123331. doi: 10.1016/j.jchromb.2022.123331. Epub 2022 Jun 14.

DOI:10.1016/j.jchromb.2022.123331

PMID:35752139

Abstract

BACKGROUND

Many guidelines and safety measures led to a decrease in exposure to antineoplastic agents. Since healthcare workers are often exposed to lower concentrations than patients, a sensitive method is needed to quantify occupational exposure.

OBJECTIVE

The aim of this study was to develop and validate a sensitive method for simultaneous detection and quantification of cyclophosphamide, ifosfamide and paclitaxel in urine by use of UPLC-MS/MS with a UniSpray ionisation source.

METHODS

Compounds were extracted from urine using Novum simplified liquid extraction cartridges, separated on a C18 column, ionised by a UniSpray ionisation source and detected with MS/MS. In the second part of the study, a field study was performed to assess occupational exposure to antineoplastic agents.

RESULTS

Eighty-three samples from healthcare workers were analysed and resulted in seventeen samples containing quantifiable concentrations of at least one compound. In conclusion, a sensitive method for simultaneous detection and quantification of cyclophosphamide (LLOQ 0.05 ng/mL), ifosfamide (LLOQ 0.3 ng/mL) and paclitaxel (LLOQ 0.7 ng/mL) was developed and validated.

摘要