Centre for Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.
Unit Environmental Hygiene and Human Biological Monitoring, Department of Health Protection, Laboratoire National de Santé (LNS), Dudelange, Luxembourg.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Aug 1;1205:123331. doi: 10.1016/j.jchromb.2022.123331. Epub 2022 Jun 14.
Many guidelines and safety measures led to a decrease in exposure to antineoplastic agents. Since healthcare workers are often exposed to lower concentrations than patients, a sensitive method is needed to quantify occupational exposure.
The aim of this study was to develop and validate a sensitive method for simultaneous detection and quantification of cyclophosphamide, ifosfamide and paclitaxel in urine by use of UPLC-MS/MS with a UniSpray ionisation source.
Compounds were extracted from urine using Novum simplified liquid extraction cartridges, separated on a C18 column, ionised by a UniSpray ionisation source and detected with MS/MS. In the second part of the study, a field study was performed to assess occupational exposure to antineoplastic agents.
Eighty-three samples from healthcare workers were analysed and resulted in seventeen samples containing quantifiable concentrations of at least one compound. In conclusion, a sensitive method for simultaneous detection and quantification of cyclophosphamide (LLOQ 0.05 ng/mL), ifosfamide (LLOQ 0.3 ng/mL) and paclitaxel (LLOQ 0.7 ng/mL) was developed and validated.
许多指南和安全措施导致接触抗肿瘤药物的暴露减少。由于医护人员经常接触到比患者低得多的浓度，因此需要一种敏感的方法来量化职业暴露。
本研究旨在开发和验证一种灵敏的方法，用于通过使用 UPLC-MS/MS 与 UniSpray 离子源同时检测和定量尿液中的环磷酰胺、异环磷酰胺和紫杉醇。
使用 Novum 简化液提取试剂盒从尿液中提取化合物，在 C18 柱上分离，用 UniSpray 离子源离子化，并用 MS/MS 检测。在研究的第二部分，进行了一项现场研究，以评估抗肿瘤药物的职业暴露。
分析了 83 名医护人员的样本，其中 17 个样本含有至少一种化合物的可定量浓度。总之，开发并验证了一种用于同时检测和定量环磷酰胺（LLOQ 0.05ng/mL）、异环磷酰胺（LLOQ 0.3ng/mL）和紫杉醇（LLOQ 0.7ng/mL）的灵敏方法。
