Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India.
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, India.
J Ethnopharmacol. 2022 Oct 5;296:115503. doi: 10.1016/j.jep.2022.115503. Epub 2022 Jun 23.
Preparations derived from the plant Calotropis procera, have been used for medicinal purpose though the plant is known for its toxic effects. The aerial parts of the plant contain latex in plenty and have been found effective in treating disorders of gastrointestinal system and cancer.
This study evaluated the efficacy of C. procera dried latex extract prepared in methanol (MeDL) against inflammation and oxidative stress in experimental model of colorectal carcinoma (CRC).
Two subcutaneous injections of chemical carcinogen, 1,2-dimethylhydrazine (DMH; 150 mg/kg) were given at an interval of one week to induce CRC in rats. The MeDL (50 and 150 mg/kg) and aspirin (60 mg/kg) were given daily and their effect was evaluated on markers of oxidative stress and inflammation after completion of 8 weeks following second injection of carcinogen. A comparison was made with normal and experimental control groups. The colon tissue levels of glutathione (GSH), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), nitrite and myeloperoxidase (MPO) were determined. Enzyme-linked immunosorbent assay was performed to determine the levels of prostaglandin E (PGE) and tumor necrosis factor-alpha (TNF-α) and immunohistochemical analysis was performed for IL-1β.
Induction of cancerous changes in the colon resulted in altered oxidative homeostasis as evident from a reduction in GSH level and SOD activity and rise in TBARS level when compared with normal rats. Elevated levels of nitrite, MPO, TNF-α, PGE and immunoreactivity of IL-1β were also observed in these rats. The levels of these markers were normalized when the rats were treated with MeDL or anti-inflammatory drug, aspirin.
This study demonstrates that suppression of oxidative stress and inflammation contributes to the beneficial effect of MeDL in rat model of colon carcinogenesis.
从植物 Calotropis procera 中提取的制剂，尽管该植物以其毒性作用而闻名，但已被用于药用目的。该植物的地上部分含有大量乳胶，并已被发现对胃肠道系统紊乱和癌症有效。
本研究评估了甲醇（MeDL）制备的 C. procera 干乳胶提取物对实验性结直肠癌（CRC）模型中炎症和氧化应激的疗效。
用化学致癌物 1,2-二甲基肼（DMH；150mg/kg）给大鼠皮下注射两次，间隔一周，诱导 CRC。MeDL（50 和 150mg/kg）和阿司匹林（60mg/kg）每天给药，并在第二次注射致癌物后 8 周完成后评估其对氧化应激和炎症标志物的影响。与正常和实验对照组进行比较。测定结肠组织谷胱甘肽（GSH）、硫代巴比妥酸反应物质（TBARS）、超氧化物歧化酶（SOD）、亚硝酸盐和髓过氧化物酶（MPO）的水平。通过酶联免疫吸附试验测定前列腺素 E（PGE）和肿瘤坏死因子-α（TNF-α）的水平，并进行白细胞介素-1β的免疫组织化学分析。
结肠癌的癌变诱导导致氧化平衡发生改变，与正常大鼠相比，GSH 水平和 SOD 活性降低，TBARS 水平升高。这些大鼠的亚硝酸盐、MPO、TNF-α、PGE 和白细胞介素-1β的免疫反应性水平也升高。当大鼠用 MeDL 或抗炎药物阿司匹林治疗时，这些标志物的水平恢复正常。
本研究表明，抑制氧化应激和炎症有助于 MeDL 在大鼠结肠癌发生模型中的有益作用。
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