Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, 600116, India.
Dept of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Mysore, Karnataka, 570015, India.
J Ethnopharmacol. 2020 Mar 25;250:112435. doi: 10.1016/j.jep.2019.112435. Epub 2019 Nov 27.
Albizia procera L. (Leguminosae) commonly known as Konda vagai in Tamil, is used for the treatment of stomach and intestinal disorders. A decoction of the bark is prescribed for rheumatism and haemorrhage. Traditionally, literature claims Albizia procera as a drug to have antirheumatic properties and hence used by Tribal for the management of chronic rheumatism. Consequently, the present study has been undertaken to illustrate the beneficial outcome of Albizia procera in adjuvant induced arthritic rat model with respect to its antioxidant and anti-inflammatory activities.
The present study is aimed to investigate the oxidative stress and the expression of inflammatory markers in arthritic rats treated with ethanolic bark extract of Albizia procera.
Ethanolic bark extract was characterized by HPTLC analysis. Acute oral toxicity study was performed according to the OECD test guideline 423 - Acute toxic class method. The anti-inflammatory effect of ETBE (100, 200 mg/kg/day/p.o.) was evaluated in complete Freund's adjuvant induced arthritic rats using diclofenac as positive control (0.3 mg/kg/day/p. o.). Plasma levels of interleukins TNF- α, IFN-α, IL-2, IL-6, myeloperoxidase and Cathepsin D levels were measured to assess the inflammatory effect of ETBE extract of Albizia procera. Further, the effect of ETBE on superoxide dismutase (SOD), glutathione peroxidase (GPX), reduced glutathione (GSH) and lipid peroxidation (LPO) were assessed in plasma.
HPTLC analysis showed the presence of 0.57% w/w of biochanin-A in ETBE. ETBE did not show any toxic signs up to 2000 mg/kg body weight. It exhibited the significant anti-inflammatory and antioxidant potential and did not show mortality up to 2000 mg/kg body weight. ETBE treatment significantly reduced the levels of TNF- α, IFN-α, IL-2, IL-6 and myeloperoxidase, and increased cathepsin D levels compared to vehicle treated animals. SOD, GSH and GPX levels were significantly restored to normal levels while LPO was significantly reduced at 200 mg/kg b. wt. Treated animals. Histopathological studies showed complete cartilage regeneration and near normal joint in ETBE treated arthritic rats.
ETBE demonstrated potent anti-inflammatory activity by modulating the expression of inflammatory cytokines and restoring the antioxidant enzyme levels.
Albizia procera L.（豆科）在泰米尔语中通常被称为 Konda vagai，用于治疗肠胃疾病。树皮煎剂用于治疗风湿病和出血。传统上，文献声称 Albizia procera 是一种具有抗风湿特性的药物，因此被部落用于治疗慢性风湿病。因此，进行了本项研究，以说明 Albizia procera 在佐剂诱导的关节炎大鼠模型中的有益作用，及其抗氧化和抗炎活性。
本研究旨在研究关节炎大鼠中 Albizia procera 乙醇树皮提取物对氧化应激和炎症标志物表达的影响。
采用 HPTLC 分析对乙醇树皮提取物进行了表征。根据 OECD 测试指南 423-急性毒性分类法进行了急性口服毒性研究。采用双氯芬酸钠作为阳性对照（0.3mg/kg/天，p.o.），评估 ETBE（100、200mg/kg/天，p.o.）对完全弗氏佐剂诱导的关节炎大鼠的抗炎作用。测量血浆中白细胞介素 TNF-α、IFN-α、IL-2、IL-6、髓过氧化物酶和组织蛋白酶 D 的水平，以评估 ETBE 提取物对 Albizia procera 的炎症作用。此外，还评估了 ETBE 对血浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、还原型谷胱甘肽（GSH）和脂质过氧化（LPO）的影响。
HPTLC 分析显示 ETBE 中含有 0.57%w/w 的大豆素-A。ETBE 高达 2000mg/kg 体重时没有显示出任何毒性迹象。它表现出显著的抗炎和抗氧化潜力，高达 2000mg/kg 体重时没有显示出死亡率。与 vehicle 处理的动物相比，ETBE 治疗显著降低了 TNF-α、IFN-α、IL-2、IL-6 和髓过氧化物酶的水平，并增加了组织蛋白酶 D 的水平。SOD、GSH 和 GPX 水平显著恢复到正常水平，而 LPO 水平在 200mg/kg b.wt. 治疗动物中显著降低。组织病理学研究表明，ETBE 治疗的关节炎大鼠完全再生了软骨，关节接近正常。
ETBE 通过调节炎症细胞因子的表达和恢复抗氧化酶水平，表现出强大的抗炎活性。
