A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland.
Department of Pathology, Kuopio University Hospital, Kuopio, Finland.
J Alzheimers Dis. 2022;88(3):949-955. doi: 10.3233/JAD-220072.
DAPI is conventionally used as a nuclear stain for cells in culture or tissue. Here we demonstrate that it binds specifically to the β-sheet core of amyloid-β plaques but not diffuse amyloid-β at the plaque periphery. The specific DAPI induced blue fluorescence is much stronger than amyloid plaque autofluorescence. DAPI staining of fibrillar amyloid deposit may yield a misleading impression of damaged or dying cells. On the other hand, it provides a handy and low-cost means of staining compact amyloid plaques together with cell nuclei in double or triple immunofluorescent studies.
DAPI 通常被用作培养细胞或组织中的核染色剂。在这里，我们证明它特异性地结合淀粉样β斑块的β-折叠核心，而不结合斑块周围的弥散淀粉样β。特异性 DAPI 诱导的蓝色荧光比淀粉样斑块自体荧光强得多。纤维状淀粉样沉积物的 DAPI 染色可能会给人一种细胞受损或死亡的错觉。另一方面，它提供了一种简便且低成本的方法，可以在双重或三重免疫荧光研究中同时对致密淀粉样斑块和细胞核进行染色。
