Department of Neurology and Psychiatry, Assiut University Hospital, Faculty of Medicine, Asyut, Egypt.
Department of Neurology and Psychiatry, Aswan University Hospital, Faculty of Medicine, Aswan, Egypt.
J Alzheimers Dis. 2022;88(3):1103-1114. doi: 10.3233/JAD-220176.
Dysbiosis of gut microbiota has been reported to be enrolled in the pathogenesis of Alzheimer's disease (AD). However, there is a lack of relevant studies on this topic in Egyptian patients with AD.
To investigate different species of gut microbiota in Egyptian patients with AD and correlate microbiota bacterial abundance with clinical data.
The study included 25 patients with AD and 25 healthy volunteers as age and sex-matched controls. Clinical data was taken for each patient, including medical history and examination; Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) were assessed for each participant. Bacterial DNA was extracted from stool, and abundance quantified via qPCR using 16S rRNA group-specific primers.
Akkermansia, Enterobacteria, Bacteroidetes, Bacillus cereus, Prevotella, and Clostridium cluster IV were more abundant in the AD group than in the control group, although there was significantly less abundance of Bifidobacterium spp., Firmicutes, and Actinobacteria in patients with AD than in controls, whereas no such significance was found for lactic acid bacteria between both groups. Lactic acid bacteria and Prevotella abundance was negatively correlated with cognitive impairment (p = 0.03 with MMSE, and p = 0.03 with MoCA). Prevotella abundance was positively correlated with age of onset and duration of illness and negatively correlated with smoking and coronary heart disease (p = 0.007, p = 0.03, p = 0.035, and p = 0.047, respectively).
The current work highlighted a significant relationship between AD and gut microbiota dysbiosis. A higher abundance of Prevotella species and lactic acid bacteria was correlated with cognition.
肠道微生物群的失调已被报道参与阿尔茨海默病（AD）的发病机制。然而，在埃及 AD 患者中，关于这一主题的相关研究较少。
研究埃及 AD 患者肠道微生物群的不同物种，并将微生物菌群细菌丰度与临床数据相关联。
本研究纳入了 25 例 AD 患者和 25 例年龄和性别相匹配的健康志愿者作为对照组。为每位患者采集临床数据，包括病史和检查；对每位参与者进行 Mini-Mental State Examination（MMSE）和蒙特利尔认知评估（MoCA）评估。从粪便中提取细菌 DNA，使用 16S rRNA 组特异性引物通过 qPCR 定量细菌丰度。
AD 组的阿克曼氏菌、肠杆菌、拟杆菌、蜡样芽孢杆菌、普雷沃氏菌和梭状芽孢杆菌 IV 比对照组更为丰富，尽管 AD 患者双歧杆菌、厚壁菌门和放线菌的丰度明显低于对照组，但两组之间的乳酸菌没有差异。乳酸菌和普雷沃氏菌的丰度与认知障碍呈负相关（p=0.03 与 MMSE，p=0.03 与 MoCA）。普雷沃氏菌丰度与发病年龄和病程呈正相关，与吸烟和冠心病呈负相关（p=0.007，p=0.03，p=0.035，p=0.047）。
目前的工作强调了 AD 与肠道微生物群失调之间存在显著关系。普雷沃氏菌和乳酸菌丰度较高与认知功能相关。
