Wang Heng, Umer Muhammad Jawad, Liu Fang, Cai Xiaoyan, Zheng Jie, Xu Yanchao, Hou Yuqing, Zhou Zhongli
State Key Laboratory of Cotton Biology /Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, China.
School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Front Genet. 2022 Jun 8;13:921096. doi: 10.3389/fgene.2022.921096. eCollection 2022.
Trichomes protect plants against insects, microbes, herbivores, and abiotic damages and assist seed dispersal. The function of genes have been found to be involved in the trichome development but the research on the underlying genetic and molecular mechanisms are extremely limited. Herein, genome wide identification and characterization of genes was performed. In total, 26 family members were identified in Gossypium species. Phylogenetic analysis, structural characteristics, and synteny analysis of s showed the conserved evolution relationships of . The promoter analysis of genes revealed hormone, stress, and development-related cis-elements. Gene ontology (GO) enrichment analysis showed that the genes were largely related to biological regulation, developmental process, multicellular organismal process. Protein-protein interaction analysis predicted several trichome development related proteins (SIM, LGO, and GRL) directly interacting with genes. Further, nine putative -miRNAs were also identified, targeting genes. RNA-Seq data of (with trichomes) and (with no trichomes) was used to perform the co-expression network analysis. was identified as a hub gene in a co-expression network analysis. RT-qPCR of gene in different tissues suggests that this gene has higher expressions in the petiole and might be a key candidate involved in the trichome development. Virus induced gene silencing of confirms its role in trichome development and elongation. Current results provide proofs of the possible role of genes and provide preliminary information for further studies of functions in trichome development.
表皮毛可保护植物免受昆虫、微生物、食草动物侵害以及非生物损害，并有助于种子传播。已发现一些基因的功能与表皮毛发育有关，但对其潜在遗传和分子机制的研究极为有限。在此，进行了全基因组范围内相关基因的鉴定和表征。在棉属物种中总共鉴定出26个该基因家族成员。对这些基因进行系统发育分析、结构特征分析和共线性分析，显示出它们保守的进化关系。对这些基因的启动子分析揭示了与激素、胁迫和发育相关的顺式作用元件。基因本体（GO）富集分析表明，这些基因主要与生物调控、发育过程、多细胞生物体过程有关。蛋白质-蛋白质相互作用分析预测了几种与表皮毛发育相关的蛋白质（SIM、LGO和GRL）可直接与这些基因相互作用。此外，还鉴定出9个假定的靶向这些基因的miRNA。利用有表皮毛和无表皮毛棉花的RNA-Seq数据进行共表达网络分析。在共表达网络分析中，某个基因被鉴定为枢纽基因。对该基因在不同组织中的RT-qPCR分析表明，该基因在叶柄中表达较高，可能是参与表皮毛发育的关键候选基因。对该基因进行病毒诱导基因沉默证实了其在表皮毛发育和伸长中的作用。目前的结果为这些基因的可能作用提供了证据，并为进一步研究其在表皮毛发育中的功能提供了初步信息。
