Department of Plant Production Technology, Faculty of Agricultural Technology, Kalasin University, Kalasin, Thailand.
Department of Science and Mathematics, Faculty of Science and Health Technology, Kalasin University, Kalasin, Thailand.
ScientificWorldJournal. 2022 Jun 15;2022:1839356. doi: 10.1155/2022/1839356. eCollection 2022.
Antibacterial activities and mode of action of Roxb extracts against methicillin-resistant (MRSA) were evaluated, with its biological properties including antioxidant activity and total phenolic content also measured. Heartwoods of Roxb were extracted by solvents including water, ethanol, acetonitrile, ethyl acetate, isopropanol, and hexane. Results showed that antibacterial activity against MRSA, antioxidant activity, and total phenolic content were highest in the acetonitrile extract. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 312.5 and 625.0 g/mL, respectively. Time-killing evaluation indicated a bactericidal mode of action with IC values against ABTS and DPPH radicals of 2.37 ± 0.09 and 32.10 ± 0.74 mg/mL, respectively, and total phenolic content 455.29 ± 18.35 mg GAE/g extract. Results suggested that acetonitrile extract of Roxb had good potential activity against MRSA, with promise for further development as a novel alternative drug.
评估了 Roxb 提取物对耐甲氧西林金黄色葡萄球菌 (MRSA) 的抗菌活性和作用模式，并测量了其生物特性，包括抗氧化活性和总酚含量。Roxb 的心材用包括水、乙醇、乙腈、乙酸乙酯、异丙醇和正己烷在内的溶剂进行提取。结果表明，乙腈提取物对 MRSA 的抗菌活性、抗氧化活性和总酚含量最高。最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 分别为 312.5 和 625.0 μg/mL。时间杀伤评估表明具有杀菌作用模式，对 ABTS 和 DPPH 自由基的 IC 值分别为 2.37±0.09 和 32.10±0.74 mg/mL，总酚含量为 455.29±18.35 mg GAE/g 提取物。结果表明，Roxb 的乙腈提取物对 MRSA 具有良好的潜在活性，有望进一步开发为新型替代药物。
