一种从人类诱导多能干细胞生成促红细胞生成素产生神经嵴细胞的方案。
A protocol for the generation of EPO - Producing neural crest cells from human induced pluripotent stem cells.
作者信息
Lavecchia Angelo Michele, Buttò Sara, Xinaris Christodoulos
机构信息
Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Bergamo, Italy.
出版信息
MethodsX. 2022 Jun 9;9:101753. doi: 10.1016/j.mex.2022.101753. eCollection 2022.
Abstract
Insufficient production of erythropoietin (EPO) leads to anaemia. Developing methods for the generation and transplantation of EPO-producing cells would allow scientists to design personalised therapeutic solutions. Here we present a simple and highly reproducible protocol for the generation of neural crest cells (NCCs) that can produce and secrete erythropoiesis-competent EPO in response to hypoxia.
摘要
促红细胞生成素（EPO）分泌不足会导致贫血。开发生成和移植产生EPO细胞的方法，将使科学家能够设计个性化的治疗方案。在此，我们展示了一种简单且高度可重复的方案，用于生成神经嵴细胞（NCCs），这些细胞能够在缺氧时产生并分泌具有促红细胞生成能力的EPO。
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