Ji Huizhuo, Zhao Wenzhu, Yu Zhipeng
School of Food Science and Engineering, Hainan University, Haikou 570228, China.
School of Food and Health, Beijing Technology and Business University, Bejing 100048, China.
Food Chem X. 2022 Jun 15;15:100366. doi: 10.1016/j.fochx.2022.100366. eCollection 2022 Oct 30.
Understanding the interaction of food derived angiotensin converting enzyme (ACE) inhibitory peptides and intestinal epithelial cell membrane may help to improve their absorption. This research aimed to study the molecular interaction of ACE inhibitory tri-peptides ADF, FGR, and MIR with DPPC membrane during absorption process. The DPPC liposome was prepared and characterized, then used as a model membrane. The permeability of tri-peptides across the membrane was investigated using Fluorescence spectroscopy. The effect of tri-peptides on the structure and dynamics of DPPC bilayers was determined using Fourier transform infrared spectroscopy. The effect of tri-peptides on the phase transition temperature in the DPPC membrane was also analyzed using Differential scanning calorimetry. The results showed that ACE inhibitory tri-peptides ADF, FGR, and MIR can penetrate into both the membrane-water interface and hydrophobic region of DPPC bilayer, and the tri-peptide FGR have higher permeability across the membrane.
了解食物来源的血管紧张素转换酶（ACE）抑制肽与肠上皮细胞膜之间的相互作用可能有助于提高它们的吸收。本研究旨在探讨ACE抑制三肽ADF、FGR和MIR在吸收过程中与二棕榈酰磷脂酰胆碱（DPPC）膜的分子相互作用。制备并表征了DPPC脂质体，然后将其用作模型膜。使用荧光光谱法研究了三肽跨膜的通透性。使用傅里叶变换红外光谱法测定了三肽对DPPC双层膜结构和动力学的影响。还使用差示扫描量热法分析了三肽对DPPC膜相变温度的影响。结果表明，ACE抑制三肽ADF、FGR和MIR可以穿透到DPPC双层膜的膜-水界面和疏水区域，并且三肽FGR具有更高的跨膜通透性。
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