磷酸化大豆肽-钙螯合物的钙结合特性、稳定性和成骨能力

Calcium-binding properties, stability, and osteogenic ability of phosphorylated soy peptide-calcium chelate.

作者信息

Kong Xiao, Xiao Ziqun, Chen Yuhang, Du MengDi, Zhang Zihui, Wang Zhenhua, Xu Bo, Cheng Yongqiang, Yu Tianying, Gan Jing

机构信息

Center for Mitochondria and Healthy Aging, College of Life Science, Yantai University, Yantai, Shandong, China.

Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.

出版信息

Front Nutr. 2023 Apr 21;10:1129548. doi: 10.3389/fnut.2023.1129548. eCollection 2023.

DOI:10.3389/fnut.2023.1129548

PMID:37153921

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10160607/

Abstract

INTRODUCTION

Bioactive peptides based on foodstuffs are of particular interest as carriers for calcium delivery due to their safety and high activity. The phosphorylated peptide has been shown to enhance calcium absorption and bone formation.

METHOD

A novel complex of peptide phosphorylation modification derived from soybean protein was introduced, and the mechanism, stability, and osteogenic differentiation bioactivity of the peptide with or without calcium were studied.

RESULT

The calcium-binding capacity of phosphorylated soy peptide (SPP) reached 50.24 ± 0.20 mg/g. The result of computer stimulation and vibration spectrum showed that SPP could chelate with calcium by the phosphoric acid group, carboxyl oxygen of C-terminal Glu, Asp, and Arg, and phosphoric acid group of Ser on the SPP at a stoichiometric ratio of 1:1, resulting in the formation of the complex of ligand and peptide. Thermal stability showed that chelation enhanced peptide stability compared with SPP alone. Additionally, results showed that SPP-Ca could facilitate osteogenic proliferation and differentiation ability.

DISCUSSION

SPP may function as a promising alternative to current therapeutic agents for bone loss.

摘要

引言

基于食品的生物活性肽因其安全性和高活性，作为钙递送载体备受关注。磷酸化肽已被证明可增强钙吸收和骨形成。

方法

引入一种源自大豆蛋白的新型肽磷酸化修饰复合物，并研究了该肽在有或无钙情况下的作用机制、稳定性和成骨分化生物活性。

结果

磷酸化大豆肽（SPP）的钙结合能力达到50.24±0.20毫克/克。计算机模拟和振动光谱结果表明，SPP可通过磷酸基团、C端谷氨酸、天冬氨酸和精氨酸的羧基氧以及SPP上丝氨酸的磷酸基团以1:1的化学计量比与钙螯合，形成配体与肽的复合物。热稳定性表明，与单独的SPP相比，螯合作用增强了肽的稳定性。此外，结果表明SPP-Ca可促进成骨增殖和分化能力。