miR-877-5p 通过靶向 FOXM1 抑制肺癌细胞进展。
MicroRNA-877-5p Inhibits Cell Progression by Targeting FOXM1 in Lung Cancer.
机构信息
Department of Respiratory and Critical Care Medicine, Changzhou Second People's Hospital Affiliated to Nanjing Medical University, Changzhou 213164, Jiangsu, China.
Department of Thoracic Surgery, Wuxi Branch of Zhongda Hospital Affiliated to Southeast University, Wuxi 214105, Jiangsu, China.
出版信息
Can Respir J. 2022 Jun 15;2022:4256172. doi: 10.1155/2022/4256172. eCollection 2022.
BACKGROUND
Many researches revealed that microRNAs (miRNAs) function as potential oncogene or tumor suppressor gene. As an antioncogene, miR-877-5p was reduced in many tumors.
OBJECTIVE
This research aimed to explore the biological role and mechanisms of miR-877-5p, which may help patients with non-small-cell lung cancer (NSCLC) find effective therapeutic targets.
METHODS
The downstream targets of miR-877-5p were predicted by Bioinformatics software. RT-qPCR and western blot were employed to analyze the gene levels. The impacts of miR-877-5p and FOXM1 were assessed by cell function experiments.
RESULTS
The miR-877-5p was reduced in NSCLC. In addition to this, it also inhibited cell progression of NSCLC cells . Moreover, the upregulation of FOXM1 expression restored the inhibitory effect of enhancement of miR-877-5p.
CONCLUSIONS
Taken together, miR-877-5p inhibited cell progression by directly targeting FOXM1, which may provide potential biomarkers for targeted therapy of NSCLC.
背景
许多研究表明 microRNAs（miRNAs）可作为潜在的癌基因或肿瘤抑制基因发挥作用。miR-877-5p 作为一种抑癌基因，在许多肿瘤中表达降低。
目的
本研究旨在探讨 miR-877-5p 的生物学功能和作用机制，以期为非小细胞肺癌（NSCLC）患者寻找有效的治疗靶点。
方法
生物信息学软件预测 miR-877-5p 的下游靶基因。采用 RT-qPCR 和 Western blot 检测基因水平。通过细胞功能实验评估 miR-877-5p 和 FOXM1 的影响。
结果
miR-877-5p 在 NSCLC 中表达降低，并且能够抑制 NSCLC 细胞的进展。此外，上调 FOXM1 的表达可以恢复 miR-877-5p 增强对细胞进展的抑制作用。
结论
miR-877-5p 通过直接靶向 FOXM1 抑制细胞进展，可能为 NSCLC 的靶向治疗提供潜在的生物标志物。
Zotero 插件