Yuni Nur Hidayati Dwi, Rully Septha, Amalia Aisyah, Larissa Widyani Elsa, Indraswari Genitri, Prasetya Adrian, Soraya Merika, Winarsih Sri, Reto Prawiro Sumarno
Department of Clinical Microbiology, Faculty of Medicine, University of Brawijaya, Malang, Indonesia.
Master Program in Biomedical Science, Faculty of Medicine, University of Brawijaya, Malang, Indonesia.
Interdiscip Perspect Infect Dis. 2022 Jun 15;2022:3751521. doi: 10.1155/2022/3751521. eCollection 2022.
Although the AMV and AMS vaccine candidates have similar characteristics as hemagglutinin and adhesive molecules, there are differences in molecular weight.
The research aims to determine the immunological cross-reaction between AMS and AMV.
Antihemagglutination test used the anti-adhesion molecular antibody AMS. Next, we examined the immune response that has to be linked with protectivity. The model of the research uses MLIL. The sample separated the mice into four groups, and each group had five mice. The first group was the negative control group. The second group was given AMV and infected with . The third group was immunized with AMV before being exposed to . The last group was infected with . The immune response results were evaluated by calculating the weight of MLIL and counting the colony of bacteria. We also examined other AMS immune responses, namely, -defensin and s-IgA levels. To get the data, we measured the number of Th17 immune effector cells, T-reg, and proinflammatory cytokine IL-17A. Data analysis was performed using ANOVA, independent -test, Kruskal-Wallis, and Mann-Whitney tests.
An antihemagglutination cross immune response, intestinal weight, the number of bacterial colonies, and other findings were found to be significant ( < 0.05) for the levels of -defensin, s-IgA, Th17, T-reg, and IL-17A.
The 49.8 kDa·MW protein subunit of the adhesion molecule could act as a candidate vaccine homologous for shigellosis and cholera in the future.
尽管AMV和AMS候选疫苗具有与血凝素和粘附分子相似的特征，但分子量存在差异。
本研究旨在确定AMS与AMV之间的免疫交叉反应。
抗血凝试验使用抗粘附分子抗体AMS。接下来，我们检测了与保护性相关的免疫反应。研究模型采用MLIL。将小鼠样本分为四组，每组五只小鼠。第一组为阴性对照组。第二组给予AMV并感染……。第三组在接触……之前用AMV免疫。最后一组感染……。通过计算MLIL的重量和计数细菌菌落来评估免疫反应结果。我们还检测了其他AMS免疫反应，即防御素和s-IgA水平。为了获取数据，我们测量了Th17免疫效应细胞、Treg和促炎细胞因子IL-17A的数量。数据分析采用方差分析、独立样本t检验、Kruskal-Wallis检验和Mann-Whitney检验。
对于防御素、s-IgA、Th17、Treg和IL-17A水平，抗血凝交叉免疫反应、肠道重量、细菌菌落数量及其他结果均具有显著性（P<0.05）。
粘附分子的49.8 kDa·MW蛋白亚基未来可能作为志贺氏菌病和霍乱的同源候选疫苗。
