Lin Lu, Pan Meng, Bezerra Jadson D P, Tian Chengming, Fan Xinlei
The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.
Setor de Micologia, Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605-050, Goiânia, Goiás, Brazil.
Plant Dis. 2023 Jan;107(1):83-96. doi: 10.1094/PDIS-02-22-0260-RE. Epub 2023 Jan 20.
Poplar is widely cultivated in China because of its strong ecological adaptability, fast growth, easy reproduction, and short rotation period. However, it suffers from severe threat from canker disease caused by species. The present study revealed the presence of species from in China. A total of six species of were isolated from in six provinces in China, including five known species (, , , , and ) and one novel species () based on morphological and phylogenetic analyses of ITS, , , , and gene sequences. , , , and are confirmed as pathogens by pathogenicity tests of which showed the strongest virulence, followed by , , and . The mycelial growth rates of isolates from the six species had 22.5 to 27°C as the optimum temperatures, and the optimum pH values were 5.9 to 7.1. The effectiveness of six carbon sources on the mycelial growth showed that colonies grew the fastest in the presence of fructose and grew the slowest using xylose. This study represents a significant evaluation of causing poplar canker disease in China.
杨树因其生态适应性强、生长快、易繁殖且轮伐期短而在中国广泛种植。然而，它受到由[某物种]引起的溃疡病的严重威胁。本研究揭示了在中国存在来自[某属]的[某物种]。基于对ITS、[其他基因名称1]、[其他基因名称2]、[其他基因名称3]和[其他基因名称4]基因序列的形态学和系统发育分析，从中国六个省份的[杨树样本]中共分离出六种[该属物种]，包括五个已知物种（[物种名称1]、[物种名称2]、[物种名称3]、[物种名称4]和[物种名称5]）和一个新物种（[新物种名称]）。通过致病性测试证实[物种名称1]、[物种名称2]、[物种名称3]和[物种名称4]为病原体，其中[物种名称1]的毒力最强，其次是[物种名称2]、[物种名称3]和[物种名称4]。六个物种分离株的菌丝生长速率表明，最适温度为22.5至27°C，最适pH值为5.9至7.1。六种碳源对菌丝生长的有效性表明，在果糖存在下菌落生长最快，而使用木糖时生长最慢。本研究对中国引起杨树溃疡病的[该属物种]进行了重要评估。
