Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Nucleosides Nucleotides Nucleic Acids. 2022;41(9):841-850. doi: 10.1080/15257770.2022.2085291. Epub 2022 Jun 27.
The association between methylation of MAOA gene promoter and alcohol and nicotine dependence has been demonstrated in women but not in men yet. Antisocial personality disorder (ASPD) and substance use disorders (SUD) are two types of disorders that could highly be influenced by methylation-induced changes in MAOA function. The aim of the current study is to investigate the effect of opioid addiction on methylation of MAOA gene promoter in males. DNA was extracted from the whole blood of all samples (29 opium-addicted individuals undergoing methadone treatment and 28 healthy people) according to the extraction protocol, followed by treating these samples with bisulfite kits. The investigated region including two CpG islands in the promoter region of MAOA gene contained 35 CpG dinucleotides investigated through Sanger sequencing method. The frequency of methylation at two CpG islands of MAOA gene promoter regions was equal to zero among addicted individuals undergoing methadone treatment and healthy peoples. Then, comparing methylation levels among the study group is not applicable. In conclusion, there was no association between opium addiction and methylation of the MAOA promoter regions in opium-addicted male undergoing methadone treatment.
MAOA 基因启动子甲基化与酒精和尼古丁依赖的相关性已在女性中得到证实，但尚未在男性中得到证实。反社会人格障碍（ASPD）和物质使用障碍（SUD）是两种受 MAOA 功能甲基化诱导变化影响很大的疾病类型。本研究的目的是探讨阿片类药物成瘾对男性 MAOA 基因启动子甲基化的影响。根据提取方案，从所有样本（29 名接受美沙酮治疗的阿片类药物成瘾者和 28 名健康人）的全血中提取 DNA，然后用亚硫酸氢盐试剂盒处理这些样本。通过 Sanger 测序法研究 MAOA 基因启动子区域内的两个 CpG 岛所包含的 35 个 CpG 二核苷酸。在接受美沙酮治疗的成瘾者和健康人群中，MAOA 基因启动子区域两个 CpG 岛上的甲基化频率均等于零。因此，不适合比较研究组之间的甲基化水平。总之，在接受美沙酮治疗的阿片类药物成瘾男性中，阿片类药物成瘾与 MAOA 启动子区域的甲基化之间没有关联。
