School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China; Pan Asia (Jiangmen) Institute of Biological Engineering and Health, Jiangmen 529080, China.
Food Chem. 2022 Nov 15;394:133545. doi: 10.1016/j.foodchem.2022.133545. Epub 2022 Jun 21.
Monascus red pigments are widely used in the food industry, mainly as intracellular red pigments. The low yields of extracellular red pigments (ERPs) make them unsuitable for large-scale industrial production. Herein, a novel integrated fermentation system (IFS) consisting of sodium starch octenyl succinate and Triton X-100 was explored for increasing yield, resulting in an ERP yield of 126.7 U/mL, 82.6% higher production than controls (69.4 U/mL). Major ERP components in control fermentations were monascopyridine A and monascopyridine B, but dehydro derivatives, rubropunctamine and monascorubramine, predominated in the test fermentations, presumably due to polyketide oxidation induced by Triton X-100. Improvement of hyphal morphology, membrane permeability, respiratory activity, and gene expression for red pigment biosynthesis is likely to be critical to increase yield and change the compositions. This study provides an effective strategy to accelerate the biosynthesis and secretion of Monascus pigments.
红曲红色素广泛应用于食品工业，主要作为细胞内红色素。细胞外红色素（ERPs）产量低，不适合大规模工业生产。在此，探索了一种由辛烯基琥珀酸钠和 Triton X-100 组成的新型集成发酵系统（IFS），以提高产量，使 ERP 产量达到 126.7 U/mL，比对照（69.4 U/mL）提高 82.6%。对照发酵中主要的 ERP 成分是 monascopyridine A 和 monascopyridine B，但在试验发酵中主要是脱氢衍生物 rubropunctamine 和 monascorubramine，这可能是由于 Triton X-100 诱导的聚酮氧化所致。菌丝形态、膜通透性、呼吸活性和红色素生物合成基因表达的改善可能对提高产量和改变成分至关重要。本研究提供了一种加速红曲色素生物合成和分泌的有效策略。
