Department of Laboratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
Department of Laboratory Medicine, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
Clin Biochem. 2022 Oct;108:56-62. doi: 10.1016/j.clinbiochem.2022.06.006. Epub 2022 Jun 24.
There is growing evidence that long non-coding RNAs (lncRNAs) play important roles in the progression of hepatocellular carcinoma (HCC) and may serve as diagnostic markers. This study investigates the diagnostic efficiency of the long intergenic non-protein-coding RNA 1793 (LINC01793) in hepatitis B virus (HBV)-related HCC.
Bioinformatics methods were used to screen the aberrantly expressed lncRNAs in HCC tissues based on The Cancer Genome Atlas (TCGA). Quantitative reverse transcription polymerase chain reaction was performed to assess the expression of the candidate lncRNAs in tissues, cells and whole blood samples of patients with HBV-related HCC, liver cirrhosis (LC), chronic hepatitis (CHB), and healthy controls. Then, the correlations between LINC01793 and clinical characteristics were analyzed. Finally, the diagnostic value of LINC01793 was explored based on the receiver operating characteristic curve.
LINC01793 was remarkably upregulated in the HCC tissues and cells. It was highly expressed in the whole blood of the HBV-related HCC patients, unlike in that of the healthy controls and of the CHB and LC patients. Subsequent analysis revealed that high LINC01793 was related to the Barcelona Clinic Liver Cancer (P = 0.007), tumor invasion (P = 0.042), the number of tumors (P = 0.031) and serum level of alanine aminotransferase(p = 0.022). The areas under the curve of LINC01793, for distinguishing HCC from healthy controls, CHB and LC patients, were 0.824, 0.767 and 0.756, respectively. In addition, the combination of LINC01793 with alpha fetoprotein (AFP) had a stronger diagnostic value than LINC01793 or AFP alone in AFP-negative HCC patients.
High expression of LINC01793 is correlated with adverse clinical characteristics and can serve as a non-invasive biomarker of HCC.
越来越多的证据表明，长非编码 RNA（lncRNA）在肝细胞癌（HCC）的进展中发挥重要作用，并可能作为诊断标志物。本研究探讨了长基因间非蛋白编码 RNA 1793（LINC01793）在乙型肝炎病毒（HBV）相关 HCC 中的诊断效率。
基于癌症基因组图谱（TCGA），使用生物信息学方法筛选 HCC 组织中异常表达的 lncRNA。采用定量逆转录聚合酶链反应（qRT-PCR）检测候选 lncRNA 在 HBV 相关 HCC 患者、肝硬化（LC）患者、慢性乙型肝炎（CHB）患者和健康对照者组织、细胞和全血样本中的表达。然后，分析 LINC01793 与临床特征的相关性。最后，基于受试者工作特征（ROC）曲线探讨 LINC01793 的诊断价值。
LINC01793 在 HCC 组织和细胞中显著上调。HBV 相关 HCC 患者的全血中高表达，与健康对照者及 CHB 和 LC 患者不同。进一步分析表明，高 LINC01793 与巴塞罗那临床肝癌（BCLC）分期（P=0.007）、肿瘤侵袭（P=0.042）、肿瘤数目（P=0.031）和血清丙氨酸氨基转移酶水平（p=0.022）相关。LINC01793 区分 HCC 与健康对照者、CHB 和 LC 患者的曲线下面积分别为 0.824、0.767 和 0.756。此外，LINC01793 与甲胎蛋白（AFP）联合检测在 AFP 阴性 HCC 患者中的诊断价值强于 LINC01793 或 AFP 单独检测。
LINC01793 的高表达与不良临床特征相关，可作为 HCC 的非侵入性生物标志物。
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