Department of Clinical Laboratory, The Public Health Clinical Center of Dalian, Dalian, Liaoning Province, China.
Turk J Gastroenterol. 2024 May;35(5):391-397. doi: 10.5152/tjg.2024.23314.
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors and the third leading cause of cancer-related mortality. Extensive literature suggests that long noncoding RNAs play a role in the progression of HCC and hold potential as diagnostic biomarkers for this disease.
We examined the serum levels of HOX antisense intergenic RNA (HOTAIR) in 49 hepatitis patients, 31 liver cirrhosis (LC), and 37 HCC patients using quantitative real-time polymerase chain reaction. Correlations between serum HOTAIR levels and clinical data were evaluated in HCC patients. The receiver operating characteristic curve was utilized to analyze the diagnostic potency of HOTAIR.
The HOTAIR levels in serum were significantly higher in HCC patients compared to those with hepatitis (P = .003) and LC patients (P = .048). There was a significant association between the serum levels of HOTAIR and positivity of hepatitis B e antigen (HBeAg) (P = .039) as well as portal vein tumor thrombus (P = .040) in HCC patients. The area under the curve (AUC) for HOTAIR for distinguishing HCC from hepatitis and LC was 0.697. The combined AUC for HOTAIR, HBeAg, and alpha-fetoprotein (AFP) was 0.777.
Serum HOTAIR functions as a potential diagnostic marker for hepatitis B virus-related HCC. Combining HOTAIR with clinical data and AFP can reinforce the diagnostic precision on HCC.
背景/目的：肝细胞癌（HCC）是最常见的恶性肿瘤之一，也是癌症相关死亡的第三大主要原因。大量文献表明，长非编码 RNA 在 HCC 的进展中发挥作用，并有可能成为该疾病的诊断生物标志物。
我们使用实时定量聚合酶链反应检测了 49 例肝炎患者、31 例肝硬化（LC）和 37 例 HCC 患者血清中 HOX 反义基因间 RNA（HOTAIR）的水平。评估了 HCC 患者血清 HOTAIR 水平与临床数据之间的相关性。使用受试者工作特征曲线分析 HOTAIR 的诊断效力。
与肝炎（P=0.003）和 LC 患者（P=0.048）相比，HCC 患者血清中的 HOTAIR 水平显著升高。在 HCC 患者中，血清 HOTAIR 水平与乙型肝炎 e 抗原（HBeAg）阳性（P=0.039）和门静脉癌栓（P=0.040）之间存在显著相关性。HOTAIR 区分 HCC 与肝炎和 LC 的曲线下面积（AUC）为 0.697。HOTAIR、HBeAg 和甲胎蛋白（AFP）的联合 AUC 为 0.777。
血清 HOTAIR 可作为乙型肝炎病毒相关 HCC 的潜在诊断标志物。将 HOTAIR 与临床数据和 AFP 相结合可以提高 HCC 的诊断精度。
